摘要
目的建立硫酸氨基葡萄糖含量测定的高效液相色谱(HPLC)法。方法样品柱前衍生化后采用反相高效液相色谱(RP-HPLC)法进行测定,色谱柱为HypersilODS柱(50mm×4.6mm,5μm),流动相为三水合乙酸钠水溶液-甲醇(90∶10),用紫外检测器检测。结果方法的进样量线性范围为300～1500μg/mL,r=0.9997(n=5),平均回收率为102.68%,RSD=1.08%(n=9)。结论此法简便、快速、准确,是测定硫酸氨基葡萄糖含量的有效方法。
Objective To establish a HPLC method for the determination of glucosamine sulphate. Methods The sample of glucosamine sul- phate was treated by pre-column derivatization, then determined by RP-HPLC on the Hypersil ODS column （50 mm ×4. 6 mm,5 μm） with CH3COONa.3H2O - CH3OH（90 ： 10） as the mobile phase and detected by the UV detector. Results The linear range of calibration curve was 300-1 500 μg/mL, r=0. 9997（n=5）.The recovery rate was 102.68%, RSD=l.08% （n=9）.Conclusion The method is simple, rapid and accurate, which is an effective method for the determination of glucosamine sulphate.
出处
《中国药业》
CAS
2010年第5期20-21,共2页
China Pharmaceuticals
