配体交换色谱手性固定相法拆分与定量茶氨酸对映体

Separation and Quantitation of Theanine Enantiomer on Ligand Exchange Chromatography with Chiral Stationary Phase

本文建立了配体交换色谱手性固定相法拆分与定量茶氨酸对映体的方法。色谱条件为:L-羟基脯氨酸键合手性柱;0.8mmol/L的硫酸铜-水溶液为流动相;波长254nm;流速1.0mL/min;柱温30℃。L-茶氨酸的进样量在0.08126～4.5144μg范围内峰面积与进样量之间呈现良好线性关系,加标回收率为97.26%～102.1%,检测限为0.02742μg,定量限为0.06503μg;D-茶氨酸的进样量在0.08250～4.5832μg范围内峰面积与进样量之间线性关系良好,加标回收率在97.07%～101.2%,检测限为0.02941μg,定量限为0.06982μg。该方法具有良好的稳定性和准确度。

Ligand exchange chromatography with chiral stationary phases was established for the separation and quantitation of theanin enantiomers.The optimum condition was：chiral column bonded with L-Hydroxypmline （250mm × 4.6mm）;mobile phase： 0.8mmol/L CuSO4-H2O;wavelength：254nm;flow rate：l.0ml./min;column temperature： 30℃ .This method showed good linearity for both L-theanine （ranging from0.08126 to 4.5144 μg） and D-theanine （ranging from0.08250 to 4.5832μg）.The recoveries were in the range of 97.26%～102.1% for L-theanine and 97.07%-101.2% for D-theanine.The resuhs demonstrated that this method was accurate.stable.