气电纺纳米羟基磷灰石-聚羟基丁酸酯复合纤维支架与大鼠成骨细胞体外生物相容性研究

Biologic Evaluation of a Novel Gas-jet/electrospun Bone Tissue Engineering Scaffolds in vitro

目的研究气电纺纳米羟基磷灰石-聚羟基丁酸酯[nanosized hydroxyapatite-poly(3-hydroxybutyrate),nHAP-PHB]复合纤维支架对大鼠成骨细胞体外粘附、增殖及骨钙素分泌活性的影响。方法实验组将大鼠成骨细胞接种于气电纺nHAP-PHB复合纤维支架进行体外培养,对照组将大鼠成骨细胞接种于气电纺纯聚羟基丁酸酯(PHB)纤维支架行体外培养。2组进行扫描电镜观察、四甲基偶氮唑盐比色检测及骨钙素检测,评价nHAP-PHB复合纤维支架对大鼠成骨细胞体外粘附、增殖以及骨钙素合成分泌活性的影响。结果扫描电镜观察结果显示接种4h后,与纯PHB纤维支架相比,细胞在nHAP-PHB复合纤维支架上的铺展更完全。培养后第3、7、10、14天,实验组细胞增殖活性均强于对照组(P<0.05),骨钙素分泌活性均高于对照组(P<0.05)。结论nHAP-PHB复合纤维中nHAP增强了支架材料与大鼠成骨细胞的生物相容性。

Objective To evaluate the biocompatibility of gas-jet/electrospun nanosized hydroxyapatite （ nHAP）/poly （3-hydroxybutyrate） （PHB）scaffolds for being used as bone tissue engineering scaffolds. Methods The attachment, proliferation and osteocalcin （OC） secretion of rat osteoblasts cultured on the nHAP-PHB scaffolds were investigated and compared with those cultured on the pure PHB scaffolds. Results PHB-based fibrous scaffolds present good ＇affinity to osteoblasts. Osteoblasts cultured on nHAP-PHB scaffolds presented better proliferation activity and OC secretion than those cultured on the pure PHB scaffolds. Conclusion The gas-jet/electrospun nHAP-PHB scaffolds present good biocompatibility and have the potential to be used in bone tissue engineering.