大黄素抑制高迁移率族蛋白B1胞外释放及其机理

Effect of emodin on extracellular release of HMGB1 and its mechanism

目的研究大黄素对脂多糖激活后巨噬细胞高迁移率族蛋白B1(HMGB1)释放的影响及其机理。方法通过细胞培养和动物实验,观察大黄素对脂多糖激活后巨噬细胞株RAW264.7HMGB1释放、HMGB1mRNA表达和HMGB1核浆转移的作用,以及对内毒素血症小鼠血清HMGB1水平和存活率的影响。结果大黄素明显抑制脂多糖激活后巨噬细胞HMGB1的基因表达、核浆转移和胞外释放,降低内毒素血症小鼠血清HMGB1水平并提高存活率。结论大黄素具有抑制HMGB1胞外释放作用。

Objective To study the effect of emodin on extracellular release of high mobility group box 1（HMGB1）from endotoxin-stimulated macrophages and its mechanism.Methods The effect of emodin was investigated on HMGB1 release,expression of HMGB1 mRNA and nuclear HMGB1 translocation using cultured macrophage-like RAW 264.7 cells after stimulation with LPS,and on serum HMGB1 level and survival rate of endotoxemia mice.The levels of HMGB1 in the culture medium or serum were determined by Western blot analysis.Results Emodin dose-dependently attenuated LPS-induced HMGB1 release from macrophage-like RAW 264.7 cells,suppressed the expression of HMGB1 mRNA and HMGB1 translocation from nuclear to cytoplasm,decreased serum HMGB1 level of endotoxemia mice,and elevated the survival rate from 35% to 55% on the 96th hour after LPS was injected in mice.Conclusion Emodin inhibits the extracellular release of HMGB1 from LPS-stimulated macrophages.