耐亚胺培南铜绿假单胞菌β-内酰胺酶相关基因及Ⅰ类整合子的检测

Detection of genes of beta-lactam antibiotics and class 1 integron from clinical isolates of imipenem-resistant Pseudomonas aeruginosa

目的研究耐亚胺培南铜绿假单胞菌(PA)β-内酰胺酶相关基因及Ⅰ类整合子的携带情况。方法采用K-B法对临床分离的PA进行药敏试验,选出26株耐亚胺培南PA,用PCR法检测其β-内酰胺酶相关基因和Ⅰ类整合子(intⅠ1和qacE△1-sul1基因)。结果26株耐亚胺培南PA体外对15种抗菌药物的药敏结果显示对多黏菌素B的敏感率为100%,对头孢哌酮/舒巴坦、哌拉西林、哌拉西林/他唑巴坦、头孢他啶、阿米卡星的敏感率分别为61.54%、61.54%、65.39%、30.77%、50.00%。对复方新诺明的耐药率为100%。16种基因检出率由高到低依次为qacE△1-sul1(92.31%),OprD2缺失(46.15%),TEM(42.31%),OXA-10(26.92%),VIM(11.76%),SPM(7.69%),VEB(7.69%),SHV(7.69%),未检出IMP、GIM、PER、GES、CARB、DHA、CTX-M、intⅠ1基因。结论耐亚胺培南PA的耐药主要与Ⅰ类整合子、OprD2缺失有关。

Objective To investigate the resistant genes of beta-lactam antibiotics and class 1 integron from clinical isolates of imipenem-resistant Pseudomonas aeruginosa（PA）.MethodsAntimicrobial susceptibilities were evaluated by disk diffusion test（K-B method）in twenty-six isolates of imipenem-resistant pseudomonas aeruginosa.Beta-lactam antibiotics and class 1 integron genes（intⅠ1 and qacE1-sul1genes）carried on imipenem-resistant Pseudomonas aeruginosa were detected by PCR method.Results Results of susceptiblity test showed that the sensitive rates of the 26 strains imipenem-resistant Pseudomonas aeruginosas to polymyxin B,cefoperazone/sulbactam,piperacillin,piperacillin/tazobactam,ceftazidime,amikacin were 100.00%,61.54%,61.54%,65.39%,30.77%,and 50.00%,respectively.The resistant rate to sulfamethoxazole/trimethoprim was 100%.The positive rate of beta-lactam antibiotics and class 1 integron genes qacE1-sul1,OprD2 defect,TEM,OXA-10,VIM,SPM,VEB,SHV were 92.31%,46.15%,42.31%,26.92%,11.76%,7.69%,7.69%,7.69%,respectively.IMP,GIM,PER,GES,CARB,DHA,CTX-M and intⅠ1 genes were not detected.Conclusion PA of our hospital resistant to imipenem is mainly related with class 1 integron genes and OprD2 gene lack mutation.