γ-分泌酶抑制剂DAPT对宫颈癌细胞系及HPV16亚基因转化的永生化人宫颈上皮细胞系作用的研究

Study on the influence of DAPT(a γ-secretase inhibitor) on cervical cancer cell lines and HPV16-transformed immortalized human cervical epithelial cell line

目的:研究γ-分泌酶抑制剂DAPT对宫颈癌细胞系以及HPV16亚基因转化的永生化人宫颈上皮细胞系的作用,探讨DAPT临床治疗宫颈癌的可行性。方法:体外培养宫颈癌Siha、HeLa细胞系和HPV16亚基因转化的永生化人宫颈上皮细胞系(CRL2614)。加入不同浓度的γ-分泌酶抑制剂DAPT,不同时间点终止细胞生长,采用四甲基偶氮唑蓝(MTT)比色方法检测细胞生长情况;使用流式细胞计数仪检测细胞增殖周期中增殖指数(PI)、S期细胞比例(SPF)和凋亡细胞百分比。结果:应用MTT法检测细胞生长情况,5μmol/L及10μmol/LDAPT作用宫颈癌细胞系72h,可显著抑制其生长(P均<0.05)。10μmol/LDAPT对CRL2614细胞系作用72h无抑制作用(P>0.05)。流式细胞计数仪检测细胞增殖周期及细胞凋亡百分比。用5μmol/L及10μmol/LDAPT作用宫颈癌细胞系24,48,72h均可见SPF、PI明显降低,凋亡细胞百分比明显增加(P均<0.05)。10μmol/LDAPT作用CRL2614细胞系72h对SPF、PI及凋亡细胞百分比无影响(P>0.05)。结论:DAPT能抑制宫颈癌HeLa、Siha细胞系增殖,促进肿瘤细胞凋亡,对HPV16亚基因转化的永生化人宫颈上皮细胞系的增殖和凋亡没有明显影响。DAPT可能为药物治疗宫颈癌提供新思路。

Objective ： To explore the influence of DAPT, a γ-secretase inhibitor on the proliferation and apoptosis in cervical cancer cell lines and HPV16-transformed immortalized human cervical epithelial cell line, and to study treatment of cervical cancer. Methods：Cervical the possibility of applying DAPT to the clinical cancer cell lines Siha and HeLa were incubated with 0.1 μmol/L, 1 μmol/L, 5 μmol/L, 10μmol/L DAPT. HPV16-transformed immortalized human cervical epithelial cell line CRL2614 was incubated with 10μmol/L DAPT. After incubation for different time, proliferation of these cells was observed by MTT assay. Cell cycle was detected by flow cytometry（ FCM ）. Results：5 μmol/L and 10μmol/L DAPT inhibited the proliferation of Siha and HeLa cells for 72h deteced by MT-F,the difference was significant（P〈0.05 ）. 10μmol/L DAPT had no effect on the proliferation of CRL2614 cells until 72h（P〉0.05 ）. Thedecrease of proliferation index（PI） and SPF and increase of apoptosis in Siha, HeLa cells and CRL2614 cells were detected by flow cytometry（FCM）. 10μmol/L and 5 μmol/L DAPT caused the decrease of PI and SPF and increase of apoptosis in Siha and HeLa cells from 24h to 72h. Compared with the control,all differences were significant（P〈0.05）. 10μmol/L DAPT had no effect on the PI,SPF and apoptosis of CRL2614 cells（P〉0.05） compared with the control. Conclusion：γ-secretase inhibitor, DAPT, may induce decreased proliferation and increased apoptosis of cervical cancer cells but has no effect on HPV16-transformed immortalized human cervical epithelial cells. DAPT gives us a new idea for the treatment of cervical cancer.