甲醛对雌性大鼠卵巢储备功能的影响

Effects of formaldehyde on ovarian reserve function in female rats

目的:在亚慢性染毒的条件下,研究甲醛对SD雌性大鼠卵巢储备功能的影响。方法:将40只SD雌性大鼠随机分为正常对照组和3个不同浓度(0.2、2.0、20.0mg/kg)的甲醛染毒组。腹腔注射甲醛染毒,每天1次,连续染毒14d,14d后称体质量,并采用股动脉放血处死受试动物,取血清,用化学发光法测定血清雌二醇(E2)浓度;用酶联免疫方法测定抑制素B(inhibinB)浓度;用放射免疫方法测定卵泡刺激素(FSH)和黄体生成激素(LH)的浓度。并解剖摘取卵巢称重,计算脏器系数。结果:甲醛染毒组与对照组比较,各染毒剂量组大鼠血清E2、InhibinB水平明显降低(P<0.05),FSH水平显著升高(P<0.05);高、中剂量组大鼠血清LH水平均有所升高,但差异无统计学意义(P>0.05)。各染毒剂量组卵巢脏器系数显著降低(P<0.05)。结论:在亚慢性染毒条件下,甲醛对SD雌性大鼠卵巢储备功能有一定损害作用。

OBJECTIVE： To explore the effects of subchronic exposure to formaldehyde on ovarian reserve function in female rats. METHODS： Forty female Spragne-Dawley （SD） rats were randomly subdivided into a control group and three formaldehyde groups. The rats in formaldehyde groups were intmperitoneally treated with formaldehyde at 0.2, 2.0 and 20.0 mg/（kg · d）for 14 days. Radioimmunoassay was used to detect estradiol（E2）, luteinizing hormone （LH）, follicle stimulating hormone （FSH） and Inhibin B. Ovary and body weights of female rots were measured and the organic coefficient of ovary was counted. RESULTS： Compared with the control group, the levels of serum estradiol （E2） and Inhibin B in the groups treated with formaldehyde decreased significantly （P 〈 0.05）. The level of serum FSH increased and the organic coefficient of ovary decreased significanfly（P 〈 0.05）, while the levels of LH didn＇t change obviously（P〉 0.05）. CONCLUSION： The ovarian reserve function of female rats can be impaired after subehronically exposed to formaldehyde for 14 days.