昆明周边部分污染水体对蚕豆根尖细胞微核率的影响

Effect of the partial polluted waters around kunming on micronucleus rate of vicia faba root tip cells

背景与目的:研究昆明周边生活区水体污染程度与致突变物质的关系。材料与方法:用蚕豆根尖细胞微核技术对各采样点水样进行监测,并对各水样进行化学需氧量(chemical oxygen demand,CODcr)的测定,统计分析各采样点水样的蚕豆根尖细胞微核千分率(MCN,‰)及污染指数(PI)。结果:各采样点水样的蚕豆根尖细胞微核率均高于对照组(P<0.01),9个采样点中有7个采样点的污染指数(PI)在2以上,污染指数高的水样组细胞微核率也高。各水样CODcr测定结果未见明显规律。结论:水体污染程度与水体中存在致突变性物质多少相关,但是水质诱变活性与化学需氧量没有直接关系。

OBJECTIVE：To examine the association between the degree of pollution of the water around residential Kunming and mutagenicity.METHODS：The micronucleus test of Vicia faba root tip cell was used to detect the water quality,and to survey the chemical oxygen demand（CODcr）.The micronuclear rates and Pollution Index（PI） were determined and the F-test was used to evaluate the difference in micronuclear rates among different samples.RESULTS：There was significant difference in micronuclear rates of every site（P〈0.01）.PI values of 7 among 9 sampling sites were over 2.00.The micronuclear rates were higher in the sites with higher PI.There was no obvious pattern in the results of CODcr.CONCLUSION：The degree of pollution was directly related to the level of mutagenicity.But the water mutagenic activity was not directly related to the chemical oxygen demand.