牛膝多糖对链佐星诱导性糖尿病小鼠的保护作用(英文)

Protective effect of Achyranthes bidentata polysaccharides on diabetic mice induced by streptozocin

目的研究牛膝多糖(ABP)对链佐星诱导性糖尿病小鼠的保护作用。方法将雄性ICR小鼠分为正常对照组、糖尿病模型组、ABP50和100mg.kg-1(ip,每天1次,共15d)治疗组。分别于给药前,给药8和15d时用血糖测量仪检测血糖水平,并进行血糖耐受试验。末次给药第2天测量小鼠体重,处死,测量心、肝、脾和肾脏器官重量,同时制备血清,采用放射免疫分析试剂盒检测血清胰岛素水平,比色法测定血清甘油三酯(TG)、总胆固醇(TC)、钙(Ca)和磷(P)含量及谷草转氨酶(GOT)、谷丙转氨酶(GPT)和碱性磷酸酶(ALP)活性。采用ELISA检测血清瘦素、脂联素、肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)的浓度。结果与正常对照组相比,糖尿病模型组小鼠体重下降,血清胰岛素水平降低,血糖升高(P<0.05,P<0.01)。ABP50和100mg.kg-1在血糖耐受试验中能较好地调节血糖水平,其血糖在15d时分别比模型组下降了27.4%和16.3%(P<0.05,P<0.01)。ABP50mg.kg-1治疗组小鼠脾和肾脏指数,血糖、血清TG和TC浓度,GOT,GPT和ALP活性均显著低于糖尿病模型组(P<0.05),血清瘦素水平下降并接近正常水平,血清脂联素、TNF-α和IL-6水平均显著高于模型组(P<0.05,P<0.01);ABP100mg.kg-1对血清TC和TG水平及ALP和GPT活性无明显作用,对上述其他指标的作用与ABP50mg.kg-1相似。各组血清胰岛素浓度及Ca和P含量均无明显变化。结论ABP对链佐星诱导的糖尿病小鼠具有一定的保护作用。

OBJECTIVE To investigate the potential protective effect of Achyranthes bidentata polysaccharides（ABP） on diabetes mice induced by streptozocin.METHODS Male ICR mice were divided into normal control,diabetes model and ABP 50 and 100 mg·kg^-1（ip,once daily for 15 d） treatment groups.On the day before ABP administration and after ABP administration for 8 and 15 d,the blood glucose content was detected with a glucometer and intraperitoneal glucose tolerance test was also conducted.After ABP administration for 15 d,the mice were sacrificed and body weight,heart,liver,spleen and kidneys weights were measured.The serum insulin concentration was determined by radioimmunoassay kit.The serum activities of glutamic oxaloacetic transaminase（GOT）,glutamic pyruvic transaminase（GPT） and alkaline phosphatase（ALP）,and serum concentrations of triglyceride（TG）,total cholesterol（TC）,calcium and phosphorus were measured by colorimetric method.Enzyme linked immunosorbent assay was employed to detect the concentrations of leptin,adiponectin,tumor necrosis factor-α（TNF-α） and interleukin-6（IL-6） in serum.RESULTS Compared with mice in normal control group,the body weight and serum insulin concentration decreased and blood glucose increased in diabetic model mice.ABP 50 and 100 mg·kg^-1 treated mice were able to normalize glucose concentrations better following a glucose tolerance test,and the blood glucose level decreased by 27.4% and 16.3%,respectively,compared with that of diabetic model mice.The relative weights of spleen and kidneys,blood glucose level,serum TG and TC concentrations,and GOT,GPT and ALP activities in mice treated with ABP 50 mg·kg^-1 were obviously lower than those of diabetes model mice.Serum leptin concentration was also markedly decreased near to normal level.However,serum concentrations of adiponectin,TNF-α and IL-6 were significantly increased comparing with diabetes model mice.ABP 100 mg·kg-1 had no obvious effect on serum TG and TC levels,and GPT and ALP activities.Its effects on the other parameters indicated above were similar to those in ABP 50 mg·kg-1 group.For the serum concentrations of insulin,calcium and phosphorus,no statistical difference could be observed among the different groups.CONCLUSION ABP possesses protective effect against streptozocin-induced diabetes in mice.