姜黄素与β-榄香稀联用对涎腺腺样囊性癌细胞株SACC-LM生长抑制和凋亡的协同作用

Inhibitory and apoptosis effect of adenoid cystic carcinoma cell line SACC-LM induced by curcumin and β-elemene

[目的]研究姜黄素与β-榄香稀联合对体外培养涎腺腺样囊性癌细胞株SACC-LM生长抑制和凋亡的情况。[方法]应用MTT测定法、流式细胞术和透射电镜观察等方法观察姜黄素协同β-榄香稀(浓度比1∶1)诱导SACC-LM细胞凋亡率及凋亡方式。[结果]1)用MTT测定法,姜黄素协同β-榄香稀(浓度比1∶1)作用SACC-LM细胞24 h后,其抑制率有明显协同效应。2)联合用药对SACC-LM肿瘤细胞Caspase-3的表达阳性率为(46.2±3.2)%明显高于单独用药组。3)用流式细胞术,10μg/mL姜黄素与10μg/mLβ-榄香稀联合作用SACC-LM细胞24 h后诱导SACC-LM细胞发生凋亡,并将细胞周期阻滞于S期。4)用透射电镜观察,协同用药(浓度比1∶1)作用SACC-LM细胞24 h后,出现明显细胞凋亡特征。[结论]姜黄素和β-榄香稀(浓度比1∶1)联合应用可增强对SACC-LM细胞株的敏感作用,并诱导其产生凋亡,使其细胞周期阻滞于S期。

[ Objective] To study the inhibitory and apoptosis effect of salivary adenoid cystic carcinoma cell line SACC - LM Induced by curcumin and β - elemene. [ Methods] Curcumin andβ - elemene（ concentration 1： 1 ） - evaluated the influence of inhibiting on cell proliferation and the apoptosis of SACC - LM cells were determined, which were checked by MTT color assay and observed with flow cytometry and electronmicroscope. [ Results ] 1 ） Using MTT test, the synergistic effect of curcumin and β- elemene could inhibit SACC - LM cells effectively after 24 hours, which did SACC - LM cells growth in a dose - dependent manner. 2） The positive expression of the Caspase - 3 of SACC - LM ceils on curcumin and β - elemene（ concentration 1 ： 1 ） much better than curcumin and β - elemene respectively. The positive expressive rate of the Caspase - 3 of SACC - LM cells on 10 μg/mL curcumin and 10 μg/mL β - elemene were （46.2 ±3.2） %. 3 ） With flow cytometry sorting,SACC- LM eells were indueed to apoptosis in 24 hours by 10 μg/mL curcumin and 10 μg/mL β -elemene. SACC - LM cells were blocked during S phase. 4 ） 10 μg/mL curcumin and 10 μg/mL β - elemene （ with 24 hours） inhibited SACC -LM cells. The trypieal ultra -struetral changes of apoptosis observed by electron -microscope was chromatin＇s cling to periphery of the shrunken nuclear. [ Conelusions] The eureumin combined with β -elemene. （eoneen- tration 1 ： 1 ） ean strengthen the sensitiveness on SACC - LM cells and induce it apoptosis. Arrest of S phase may induce apoptosis of SACC - LM cells.