p53基因cDNA突变及其蛋白高表达与大肠癌预后关系

p53 cDNA MUTATION AND P53 PROTEIN OVEREXPRESSION IN PROGNOSIS OF COLON CANCER

１００份大肠癌组织，用ＲＴ－ＰＣＲ－ＳＳＣＰ检测ｐ５３基因ｃＤＮＡ突变；ＰＡｂ１８０１单抗免疫组化检测ｐ５３基因蛋白高表达并对大肠癌术后病人作５年生存随访，比较上述２结果与大肠癌预后的关系。１００例大肠癌中，ＲＴ－ＰＣＲ－ＳＳＣＰ显示５１例大肠癌ｐ５３基因ｃＤＮＡ突变（５１％），ＰＡｂ１８０１阳性率６２％。ｐ５３基因ｃＤＮＡ突变和ｐ５３基因蛋白高表达与Ｄｕｋｅｓ分期无关，ｐ５３基因ｃＤＮＡ突变与ｐ５３基因蛋白高表达相关（Ｐ＜０．０５）。结果提示，ＰＡｂ１８０１单抗检测ｐ５３基因蛋白高表达与大肠癌预后无关；ｐ５３基因ｃＤＮＡ突变与大肠癌预后相关（Ｐ＜０．０５）。ｐ５３基因ｃＤＮＡ突变与ｐ５３基因蛋白高表达直接相关（Ｐ＜０．０５）。ｐ５３基因ｃＤＮＡ突变是大肠癌预后差的分子生物学标志物（Ｐ＜０．０５）。ｐ５３基因蛋白产物受多种因素影响不能反映大肠癌的预后。

To study the prognostic significance between p53 cDNA mutation measured with RT PCR SSCP and P53 protein overexpression by PAb 1801 monoclonal antibody in 100 cases of colorectal adenocarcinomas. The follow up survey of all patients was done during 5 years, and the relation between p53 cDNA mutation and P53 protein overexpression with the prognosis of colon cancer was assessed. 51 cases of p53 cDNA mutations (51%) were detected by RT PCR SSCP and 62 cases of P53 protein overexpression (62%) were shown by PAb 1801 monoclonal antibody immunohistochemistry staining in 100 cases of colorectal adenocarcinomas. There was no relationship between p53 cDNA mutation and P53 protein overexpression with Dukes' stage ( P >0.05). The data showed that whereas p53 cDNA mutation was associated with P53 protein overexpression ( P <0.05), and p53 cDNA mutation did provide prognostic information ( P <0.05), while P53 protein overexpression did not. p53 cDNA mutation could provide poor prognostic information and was the molecular biomarker of poor survival of colon cancer ( P <0.05), and P53 protein overexpression could not, and might be interfered by multiple factors existing in colon cancer.