摘要
Calponin是一种抗细胞增殖基因,在多种病理情况下表达失调。用脂质体+载体质粒pCAGGS作介导向培养的自发性高血压大鼠(SHR)血管平滑肌细胞内导入人重组Calponin(hCN),目的基因可在该细胞组织内表达,并持续较长时间(4周以上)。MTT法的检测结果表明,导入hCN基因能明显抑制SHR平滑肌细胞的增殖。这种作用对血管成形术后再狭窄的防治和高血压时血管壁肥厚的逆转具有潜在的实用价值。
Calponin is a kind of gene that can suppress the proliferation of cells, and it may express abnormally under some pathological conditions. We transferred the human recombinant Calponin (hCN) into the cultured vascular smooth muscle cells (VSMC) of spontaneously hypertensive rats (SHR) with the mediation of liposome + pCAGGS. hCN could be expressed in cultured SHR VSMC for more than 4 weeks. MTT method was used to detect the proliferation of VSMC. The results revealed that the proliferation of cultured SHR VSMC could be significantly suppressed by the transfer of hCN. It was suggested that Calponin could play a role in the prevention and treatment of re stenosis after angioplasty and in the recovery of vascular wall hyperplasia during hypertension.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1998年第6期425-427,共3页
Medical Journal of Chinese People's Liberation Army