Calponin对培养的自发性高血压大鼠血管平滑肌细胞增殖的作用

THE EFFECT OF CALPONIN ON THE PROLIFERATION OF CULTURED SHR VASCULAR SMOOTH MUSCLE CELLS

Ｃａｌｐｏｎｉｎ是一种抗细胞增殖基因，在多种病理情况下表达失调。用脂质体＋载体质粒ｐＣＡＧＧＳ作介导向培养的自发性高血压大鼠（ＳＨＲ）血管平滑肌细胞内导入人重组Ｃａｌｐｏｎｉｎ（ｈＣＮ），目的基因可在该细胞组织内表达，并持续较长时间（４周以上）。ＭＴＴ法的检测结果表明，导入ｈＣＮ基因能明显抑制ＳＨＲ平滑肌细胞的增殖。这种作用对血管成形术后再狭窄的防治和高血压时血管壁肥厚的逆转具有潜在的实用价值。

Calponin is a kind of gene that can suppress the proliferation of cells, and it may express abnormally under some pathological conditions. We transferred the human recombinant Calponin (hCN) into the cultured vascular smooth muscle cells (VSMC) of spontaneously hypertensive rats (SHR) with the mediation of liposome + pCAGGS. hCN could be expressed in cultured SHR VSMC for more than 4 weeks. MTT method was used to detect the proliferation of VSMC. The results revealed that the proliferation of cultured SHR VSMC could be significantly suppressed by the transfer of hCN. It was suggested that Calponin could play a role in the prevention and treatment of re stenosis after angioplasty and in the recovery of vascular wall hyperplasia during hypertension.