锌对体外培养人胚成骨细胞生长影响的实验研究

EFFECT OF ZINC ON HUMAN EMBRYO OSTEOBLAST CULTURED IN VITRO——AN EXPERIMENTAL STUDY

采用体外培养人胚成骨细胞模型，检测加入不同浓度的锌（１０－７～１０－３ｍｏｌ／Ｌ），对人胚成骨细胞增殖和分化的影响。结果显示，锌在１０－４ｍｏｌ／Ｌ浓度时，能够显著促进成骨细胞蛋白质的合成，提高碱性磷酸酶（ＡＬＰ）活性。但对成骨细胞增殖作用不明显。当锌浓度低于１０－６ｍｏｌ／Ｌ时，作用不明显；当锌浓度超过１０－４ｍｏｌ／Ｌ，达到１０－３ｍｏｌ／Ｌ时，可抑制成骨细胞的分化，使ＡＬＰ活性下降，蛋白质合成减少。

With human embryo osteoblast cultured in vitro, the effects of zinc in different concentrations(10 -7 ￣10 -3 mol/L) on human embryo osteoblast proliferation, protein sysnthesis and activity of alkaline phosphatose (ALP) were tested. The results revealed: (1)Zinc of 10 -4 mol/L could significantly promote the protein synthesis in osteoblast and increase ALP activity, without affecting the osteoblast proliferation; (2)Zinc below 10 -6 mol/L did not show effect; (3)Zinc above 10 -4 could inhibit osteoblast differentiation and reduce ALP activity and protein synthesis.