人大肠癌组织中nm23-H_1表达及其与转移的关系

. Relationship between the expression of nm23 H1 gene and metastasis of human colorectal carcinoma

目的：探讨ｎｍ２３－Ｈ１基因表达与大肠癌转移的关系。方法：应用过氧化酶标记的链霉卵白素（ＳＰ）染色法对３３例正常大肠粘膜、１３４例癌旁粘膜及１９３例大肠癌组织进行免疫组织化学染色，并对染色结果进行观察。结果：大肠粘膜与癌旁粘膜间ｎｍ２３－Ｈ１表达阳性率差异无显著性（Ｐ＞０．０５），但ｎｍ２３－Ｈ１在良、恶性大肠癌组织中的表达阳性率差异有显著性（Ｐ＜０．０５～０．０１）。ｎｍ２３－Ｈ１表达阳性率仅在正常、良性大肠组织与转移的大肠癌间差异有显著性（Ｐ＜０．０１），但与未转移大肠癌间差异无显著性（Ｐ＞０．０５）。在已发生转移的大肠癌组织中，ｎｍ２３－Ｈ１表达阳性率和强度明显低于尚未发生转移者（Ｐ＜０．０１）。在３９例无淋巴结转移的大肠癌组织中未见ｎｍ２３－Ｈ１表达，而在２４例转移的大肠癌中可检测到ｎｍ２３－Ｈ１表达。结论：ｎｍ２３－Ｈ１在抑制大肠癌转移中发挥一定作用，但在大肠癌转移过程中可能还有其他调节途径／因素存在。

Objective: To explore the relationship between the expression of nm23 H 1 gene and metastasis of human colorectal carcinoma. Methods: Expression of nm23 H 1 in 327 cases of human benign and malignant colorectal tissues and in 33 cases of normal large bowel mucosas was investigated with S P immunohistochemical staining. Results: The incidences of nm23 H 1 expression in normal mucosas,paracancerous mucosas and carcinomas were 63.3% (21/33), 63.4% (85/134) and 39.3% (76/193) respectively.Significant difference was only found between the level of nm23 H 1 in the colorectal carcinoma group with lymph node metastasis and that of the normal/benign group ( P <0.01). The expression level of nm23 H 1 gene in the colorectal carcinoma group with lymph node metastasis was markedly lower than that of the group without lymph node metastasis ( P <0.01). There was no nm23 H 1 expression in 39 cases of colorectal carcinoma group without lymph node metastasis, however , and nm23 H 1 expression in 24 cases of the group with metastasis was detected. Conclusions: nm23 H 1 has some inhibitory effect on the metastasis of colorectal carcinoma,and there may be some other regulation paths or factors which also play certain role in the metastasis of colorectal carcinoma besides nm23 H 1.