摘要
目的：通过对慢性砷中毒大鼠脊髓神经丝蛋白（ＮＦ）的免疫组化观察，研究其脊髓神经元的病理学改变。方法：采用实验组和对照组，分别于大鼠砷中毒３５ｄ、３个月、６个月时取材，进行免疫组化研究。结果：中毒３５ｄ脊髓内ＮＦ免疫反应基本同对照组；中毒３个月脊髓内ＮＦ阳性反应强度明显低于对照组；中毒６个月ＮＦ阳性反应产物明显减少或消失。结论：实验表明砷中毒可影响ＮＦ的合成，且其毒性具有蓄积性。
Objective: The pathological changes of motorneurons were studied by observation of the neuorofilament (NF) of the spinal cords of the rats with chronic arseniasis immunohistochemically. Methods: The rats were divided into experimental group and control group at random and were sampled in 35 days 3 and 6 months with immunohistochemical study. Results: After poisoning, in 35 days the NF postive reaction was not different from control group; in 3 months it was significantly lower and in 6 months was significantly decreased or disappeared. Conclusions: Experiment shows that arseniasis could effect the synthesis of neurofilament protein and the poisoning of arsenic could be stored up. Arsenic could cause direct damages to neuron in spinal cord.
关键词
砷中毒
神经丝蛋白
脊髓
大鼠
arseniasis
neurofilament protein
rats
spinal cord
immunohistochemical
