PTEN和FHIT在上皮性卵巢肿瘤中的表达及其临床意义

Expression levels and clinical significance of PTEN and FHIT in epithelial ovarian tumors

目的:探讨PTEN和FHIT蛋白在上皮性卵巢肿瘤中的表达及意义。方法:收集该院85例上皮性卵巢肿瘤的妇科手术石蜡标本(51例卵巢癌、16例卵巢交界性肿瘤、18例卵巢良性肿瘤),另收集同期其他条件类似的10例正常卵巢组织作对照,采用免疫组织化学SP法检测组织中FHIT和PTEN表达。结果:FHIT在10例正常卵巢组织、18例良性上皮性卵巢肿瘤和16例交界性肿瘤中均呈阳性表达,在上皮性卵巢癌中,其阴性表达率(37.3%,19/51)明显高于良性卵巢肿瘤和交界性卵巢肿瘤,其差异均有统计学意义(P<0.05)。FHIT蛋白阴性表达率在伴淋巴结转移组和不伴淋巴结转移组比较差异也有统计学意义(P<0.05);FHIT蛋白的阴性表达率与病理类型及临床分期无关。卵巢交界性肿瘤和卵巢癌组织中PTEN蛋白表达显著低于卵巢良性肿瘤和正常卵巢组织,其差异均有统计学意义(P<0.05);PTEN蛋白表达与卵巢癌组织分化程度呈正相关(P<0.01),与临床病理分期负相关(P<0.05)。结论:PTEN基因和FHIT基因表达降低或缺失在卵巢癌的发生、侵袭和转移中可能起重要作用,PTEN和FHIT联合检测有助于卵巢肿瘤的早期诊断、早期治疗及生物学行为的评估。

Objective： To explore the expression levels and clinical significance of PTEN and FHIT in epithelial ovarian tumors. Methods： Surgical specimens in paraffin of 95 cases （51 cases of ovarian cancer, 16 cases of borderline ovarian tumor, 18 cases of benign ovarian tumors, 10 cases of normal ovarian tissue） were collected, the expression levels of FHIT and PTEN were detected by immunohistochemical SP method. Results： FHIT expressed in 10 cases of normal ovarian tissue, 18 cases of benign ovarian tumor and 16 cases of borderline ovarian tumor, the negative expression rate in ovarian cancer was significantly higher than those in benign ovariar/tumor and borderline ovarian tumor （ P 〈 0. 05 ） . There was significant difference in negative expression rate of FHIT protein between lymphatic metastasis group and non - lymphatic metastasis group （ P 〈 0. 05 ） ; negative expression rate of FHIT protein was not related to pathological types and clinical staging. The expression levels of PTEN protein in borderline ovarian tumor and ovarian cancer were significantly lower than those in benign ovarian tumor and normal ovarian tissue （ P 〈 0. 05 ） . There was a positive correlation between PTEN protein expression and differentiation grades of ovarian cancer （P 〈 0. 01 ）, and there was a negative correlation between PTEN protein expression and clinical pathological staging （ P 〈 0. 05 ） . Conclusion ： Low expression levels and expression deletion of PTEN gene and FHIT gene play an important role in the oncogenesis, invasion and metastasis of ovarian cancer, combined detection of PTEN and FHIT contribute to early diagnosis, early treatment and assessment of biological behaviors.