三七对酒精性肝病大鼠血清TNF-α IL-6 IL-8水平的影响

The Level of TNF-α IL-6 IL-8 in Serum of Alcoholic Hepatopathy Rats and Assess the Effects of Notoginseng

目的:观察三七对酒精性大鼠血清TNF-α、IL-6、IL-8水平的影响。方法:SD雄性大鼠随机分为正常组,模型组、三七高、低剂量组和硫普罗宁组,连续14周建立酒精性肝病模型。在模型制备同时,每天下午分别灌服给药,连续14周。ELISA法检测肿瘤坏死因子(TNF-α),放射免疫法测定血清IL-6、IL-8水平。常规HE及Masson染色,光镜观察肝组织的脂肪变、炎症化程度。结果:酒精性肝病模型组大鼠肝组织脂肪变及炎症程度计分、血清TNF-α、IL-6、IL-8水平明显增高(P<0.01,P<0.05);三七高、低剂量组,硫普罗宁组大鼠肝组织脂肪变及炎症程度、血清TNF-α、IL-6、IL-8水平较模型组明显减轻(P<0.01,P<0.05)。相关分析显示:血清TNF-α水平与肝组织炎症程度计分呈正相关(r=0.60,P<0.01),血清IL-6水平与肝组织炎症程度计分呈正相关(r=0.47,P<0.01)血清IL-8水平与肝组织炎症程度计分呈正相关(r=0.59,P<0.01)。结论:三七可明显减轻酒精性肝病大鼠肝组织脂肪变和炎症程度。三七能调节细胞因子网络,抑制ALD大鼠血清TNF-α、IL-6、IL-8的生成;这可能是其有效防治酒精性肝病的发生发展的重要机制之一。

Objective： To investigate the level of TNF-α,IL-6,IL-8 in serum of Alcoholic Hepatopathy Rats and assess the effects of Notoginseng.Methods： SD male rats were randomly divided into five groups： normal group,control group,high-dose Notoginseng roup,low-dose Notoginseng group,and Tiopronin group.All rats were induced alcoholic hepatopathy in 14 weeks and were treated every afternoon during the 14-week respectively.The TNF-α of blood were determined by ELISA.The IL-6,IL-8 of blood were determined by radioimmunoassay.Results： Compared with rats from the normal group,rats in the control group have significantly higher steatosis,inflammation,rats in the control group have significantly higher level of TNF-α,IL-6,IL-8 in blood（P0.01,P0.05）,but treatment with high-dose,low-dose Notoginseng,or Tiopronin resulted in lower level（P0.01,P0.05）.Results from the correlation analysis suggested that TNF-α is positively associated with liver inflammation（r=0.60,P0.01）.Similarly,IL-6 is positively correlated with liver inflammation（r=0.47,P0.01） finally,IL-8 is positively correlated with liver inflammation（r=0.59,P0.01）.Conclusion： Notoginseng can significantly degrade the level of steatosis,inflammation,TNF-α,IL-6 and IL-8 in the alcoholic hepatopathy rats.