维生素E和硒互作对凡纳滨对虾(Litopenaeus vannamei)抗氧化系统的调节作用

EFFECTS OF DIETARY VITAMIN E AND SELENIUM ON THE ANTIOXIDANT SYSTEM OF SHRIMP LITOPENAEUS VANNAMEI

采用二因素三水平设计试验,用添加不同水平配比的VE和Se(mg/kg)的饵料G0(0,0)、G1(200,0.2)、G2(200,0.4)、G3(200,0.8)、G4(400,0.2)、G5(400,0.4)、G6(400,0.8)、G7(800,0.2)、G8(800,0.4)、G9(800,0.8)投喂凡纳滨对虾,研究VE和Se对体长3cm左右凡纳滨对虾体液抗氧化系统的影响,试验进行4周。结果表明,第2周时,VE、VE和Se对O2?、酚氧化酶(PO)、血清总抗氧化能力(T-AOC)有显著影响(P<0.05);Se对PO、血清和肝胰腺中T-AOC以及肝胰腺中GPx有显著影响(P<0.05)。第4周时,VE和Se协同作用对4种抗氧化指标都有显著影响(P<0.05)。第2、4周时,抗氧化酶活力比第0周显著升高。对O2?的研究发现,在第2和第4周时,G8组血细胞O2?最多。第2周时,血清PO在G5、G9组活力最高(P<0.05),两组间差异不显著(P>0.05);血清和肝胰腺中T-AOC在G2、G3组最高(P<0.05);对虾肝胰腺中GPx酶活力在G3和G5组达到最高(P<0.05),两组间差异不显著(P>0.05)。第4周时,G7组PO和GPx活力最高,G9组T-AOC活力达到最高(P<0.05)。研究结果提示:添加适量的VE和Se能显著提高对虾抗氧化能力,VE和Se对抗氧化系统具有随时间变化的动态调节作用,VE和Se之间存在交互作用。当在基础饵料中分别添加VE和Se在400mg/kg、0.4mg/kg时,凡纳滨对虾机体抗氧化能力整体达到平衡,能有效抵制氧自由基的损伤。

Litopendeus vannamei （PL 3cm） samples were divided randomly into 10 groups in triplication, each contained 30 individuals. A matrix of three-level by two-factor was designed. The shrimp were fed with vitamin E and selenium for each group, respectively as： Go （0, 0）, G1 （200, 0.2）, G2 （200, 0.4）, G3 （200, 0.8）, G4 （400, 0.2）, G5 （400, 0.4）, G6 （400, 0.8）, G7 （800, 0.2）, G8 （800, 0.4）, and G9 （800, 0.8mg/kg）. The experiment lasted for four weeks during which the effects of different levels of vitamin E and selenium on O^2- in haemocytes, Phenoloxidase （PO） activity and total-antioxidative activity （T-AOC） in serum and hepatopancreas, and glutathione peroxidase （GPx） activity in hepatopancreas, were studied. The antioxidant activity was checked at 0, 2, and 4 weeks. The result shows that vitamin E and selenium could enhance the antioxidant ability dynamically. The optimal point of adding scheme was at 400mg/kg vitamin E and 0.4mg/kg selenium into the basal diets, at which the overall antioxidant system of the shrimp would reach the balance for fighting against the damage by oxygen radical.