极地寡营养细菌(Alteromonas stellipolaris sp. nov.)色素的提取及其理化性质的研究

EXTRACTION AND CHEMO-PHYSICAL CHARACTERISTICS OF BROWN PIGMENT PRODUCED BY OLIGOTROPHIC BACTERIUM ALTEROMONAS STELLIPOLARIS sp. nov. IN POLAR SEA

以分离自南极海洋的寡营养细菌所产的褐色素为试验材料,进行了该色素的提取和稳定性分析。结果表明,色素粗品至少含有3种以上不同的褐色素,该褐色素易溶于水和甲醇;最大吸收波长为214nm;在pH≥4.0、热、光照(自然光与紫外线)下都能表现出良好的稳定性;抗H2O2、HNO3氧化能力强,NaClO对其破坏较大;Na2SO3、抗坏血酸对其有护色作用;Fe3+、Fe2+和Pb2+则有一定的破坏作用,Mg2+对该色素有护色作用,其它金属离子影响不大。试验表明,寡营养细菌褐色素在稳定性上呈现出一定优势,具有作为一种天然色素资源的开发和应用价值。

The extraction condition and stability of brown pigment from oligotrophic bacteria Alteromonas stellipolaris sp. nov. collected from deep sea of the Antarctica are studied. The results show that at least three types of brown pigments were found in the crude pigment, and they are easy to dissolve in water and methanol. The maximum absorption wavelength is 214nm. The pigment was stable in pH ≥ 4.0, heat, and illumination （natural light or ultraviolet radiation） conditions, unstable in NaClO, and strong against H2O2 and HNO3. Na2SO3 and ascorbic acid were protective to the pigment. Fe^3＋, Fe^2＋, Pb^2＋, but Mg^2＋ solution was protective to it. Other metals had weak effects on the pigment. Therefore, the brown pigment of Alteromonas stellipolaris sp. nov. is quite stable and can be developed for future application.