阿托伐他汀影响原发性高血压患者循环血单个核细胞炎症因子表达的临床研究

Effects of Atorvastatin on Secret Inflammatory Cytokine in Peripheral Blood Mononuclear Cells From Essential Hypertension Patients

目的探讨阿托伐他汀对原发性高血压(EH)患者循环血单个核细胞(PBMC)分泌炎症细胞因子——肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、白细胞介素1β(IL-1β)的影响。方法40例血脂正常的EH男性患者随机分成阿托伐他汀治疗组(阿托伐他汀10 mg.d-1,qd,20例)及20例常规治疗组治疗3个月。采用密度梯度离心法分离PBMC,检测治疗前后PBMC分泌TNF-α、IL-6、IL-1β水平,20名年龄与性别相匹配的健康者作为正常对照。结果EH患者PBMC分泌TNF-α、IL-6、IL-1β水平明显高于正常对照组(P<0.01);治疗后PBMC分泌TNF-α、IL-6、IL-1β水平,阿托伐他汀组明显低于治疗前(P<0.01、P<0.05),常规治疗组则无显著差异(P>0.05)。两组治疗前后血压有显著差异(P<0.01),但组间无显著差异(P>0.05)。结论EH患者PBMC处于激活状态,存在炎症反应。阿托伐他汀可抑制PBMC分泌炎症细胞因子,可能有利于改善并延缓高血压的进程。

OBJECTIVE To investigate the effects of atorvastatin on secret inflammatory cytokine-tumor necrosis factor-alpha （TNF-ct）, interleukin-6 （IL-6） and interleukin-1 [5（ IL-1 [3） in peripheral blood mononuclear cells（PBMC） from essential hypertension （EH） patients. METHODS Forty male patients with EH were divided randomly into two groups ： atorvastatin therapeutic group （ an- tihyper-tensive therapy ＋ atorvastatin 10 mg.d^-1 for 6 months） and conventional therapeutic group. The levels of TNF-α, IL-6 and IL-1β in PBMC were measured before and after 3 months. 20 age-and sex-nlatehed healthy subjects served as controls. RESULTS The TNF-a, IL-6 and IL-1β levels of PBMC in EH patients were significantly higher than those of normal controls（P 〈 0. 01 ）. TNF-α, IL- 6 and IL-1 β levels of PBMC after treatment were significantly lower than before in atorvastatin therapeutic group （P 〈 0. 05, P 〈 0. 01 ）. There were not significant differences between before and after treatment in conventional therapeutic group（P 〉 0. 05）. CON- CLUSION The activated PBMC may be involved in the pathogenesis of EH.