不同采收期刺五加叶与无梗五加叶活性成分分析

Analysis of the Active Ingredient of the Leaves in Different Collecting Time of Acanthopanax senticosus and Acanthopanax sessiliflorus

目的探讨刺五加叶与无梗五加叶的最佳采收期,比较二者金丝桃苷、紫丁香苷及微量元素含量的差异,从而区分两个相近种的差异,并讨论二者混用的弊端。方法采用高效液相色谱法测定金丝桃苷及紫丁香苷含量,采用原子吸收光谱法测定微量元素含量,采用SPSS16.0软件对测定结果进行统计分析。结果刺五加叶中紫丁香苷与金丝桃苷含量明显高于无梗五加叶,且在6月21日和7月6日达到最高值,分别为0.3111%与0.6868%。无梗五加叶中微量元素含量高于刺五加叶,其中,铁在6月5日达到最高值,锰、锌、铜在6月21日达到最高值。结论刺五加叶的最佳采收期为6月21日至7月6日,无梗五加叶的最佳采收期为6月5日至6月21日。二者活性成分含量存在显著差异,刺五加叶适于药用,而无梗五加叶适于食用。

OBJECTIVE To evaluata the optimum collecting time of Acanthopanax senticosus leaves and Acanthopanax sessiliflorus leaves, and compare the differences of hyperin, syringin and trace elements, in order to distinguish between the two similar species and discuss the disadvantages of confusion. METHODS The contents of hyperin and syringin were determined by HPLC, and the contents of trace elements were determined by flame atomic absorption spectrophotometry. The statistical analysis was performed with SPSS16.0 . RESULTS The content of hyperin and syringin in Acanthopanax senticosus leaves showed apparently higher than that in Acanthopanax sessiliflorus leaves. The highest contents of syringin reached 0. 311 1% on June 21st and hyperin reached 0. 686 8% on July 6th. The contents of trace elements in Acanthopanax sessiliflorus leaves were apparently higher than that in Acanthopanax senticosus leaves. The contents of iron reached the highest point on June 5th. The contents of manganese, zinc and copper reached the highest content on June 21st. CONCLUSION The optimum collecting time of Acanthopanax senticosus leaves is from June 21st to July 6th, while Acanthopanax sessiliflorus leaves is from June 5th to June 21st. The discrepancy of active ingredient between them are obvious, so that they are not suitable for being mixed. The Acanthopanax senticosus leaves are suitable for medicinal materials, and the Acanthopanax sessiliflorus leaves are suitable for food.