摘要
目的建立测定他克莫司眼用乳状液含量的高效液相色谱法。方法采用DiamonsilC18（4．6mm×250mm，5μm）为色谱柱；流动相为乙腈．水-磷酸（600：400：1）；流速为2．0mL·min^-1；检测波长为215nm；柱温为50℃。结果他克莫司在6．25～200mg·L^-1。内线性关系良好（r=0．9999），平均回收率为99．35％，RSD为0．81％，方法重现性的RSD为1．04％（n=6）。结论本方法专属性强、操作简便、结果准确，可用于测定他克莫司眼用乳状液的含量。
OBJECTIVE To establish a HPLC method for the content determination of tacrolimus ophthalmic emulsion. METHODS The separation was performed on a Diamonsil C18 column（4. 6 mm × 250 mm,5 μm）. The mobile phase consisted of acetonitrile- water- phosphonic acid （600：400：1 ） at the flow of 2. 0 mL · min^-1. The detection wavelength was at 215 nm and column temperature was 50 ℃. RESULTS The calibration curve of tacrolimus was linear within the concentration range of 6. 25 - 200 mg· L^-1 （ r =0. 999 9）.The average recovery of tacrolimus was 99.35% （ RSD =0. 81% ）, and the RSD of the repeatability test was 1.04% （ n = 6）. CONCLUSION The method is simple, quick and specific. It can be used to determine the content of tacrolimus ophthalmic emulsion.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2010年第3期221-223,共3页
Chinese Pharmaceutical Journal
