藏药材印度獐牙菜质量标准研究

Quality Control of Traditional Tibetan Medicine Swertia chirayita

目的建立印度獐牙莱药材的质量标准。方法采用薄层色谱法鉴别印度獐牙菜药材中獐牙莱苦苷、龙胆苦苷和芒果苷成分；以高效液相色谱法测定该3种成分的含量。色谱柱为ZorbaxEclipseXDB—C18（4．6mm×250mm，5μm），流动相为甲醇-水（0．1％冰醋酸）线性梯度洗脱，甲醇体积分数从22％经18min至32％并保持5min，流速为1．0mL·min^-1，柱温为25℃，检测波长为243nm。结果印度獐牙菜药材中獐牙菜苦苷、龙胆苦苷和芒果苷的薄层色谱鉴别特征明显，专属性强；药材中獐牙菜苦苷、龙胆苦苷和芒果苷的含量测定线性范围分别为0．06～2．00μg（r=0．9997），0．09—2．90μg（r=0．9997）和0．05～1．65μg（r=0．9998），平均回收率（n=9）分别为103．76％（RSD=1．34％），99．43％（RSD=1．60％）和96．22％（RSD=1．30％）。结论所建立的印度獐牙菜定性定量测定方法简单准确，能够为印度獐牙菜药材的质量控制提供有效数据．

OBJECTIVE To establish a method of quality control of Swertia chirayita （ Roxb. ex Fleming） Karsten. METHODS Swertiamain, gentiopicrin and mangiferin was identified by TLC in Swertia chirayita （Roxb. ex Fleming） Karsten. Its content was determined by HPLC equipped with, Zorbax Eclipse XDB-C18 （4. 6 mm× 250 mm ,5 μm）column. The mobile phase consisted of meth- anol-water（ 0. 1% glacial acetic acid）, which concentration of methanol from 22% to 32% in 18 rain, then kept for 5 min. The anlysis was done at a flow rate of 1.0 mL · min^-1. The column temperature was 25 ℃ and the detection wavelength was 243 nm. RESULTS The linearities of swertiamain, gentiopicrin and mangi%rin were in the ranges of 0.06 - 2.00 μg （ r = 0. 999 7 ） , 0.09 - 2.90 μg （ r = 0. 999 7） and 0. 05 - 1.65 μg（ r = 0. 999 8）, respectively. The average recoveries （ n = 9） were 103.76% （ RSD = 1.34% ） for swertiamain,99. 43% （ RSD = 1.60% ） for gentiopicrin and 96.22% （ RSD = 1.30% ） for mangiferin. CONCLUSION This method is simple, accurate with good reproducibility. It is suitable for quality control of Swertia chirayita（ Roxb. ex Fleming） Karsten.