胰岛素对高血压大鼠局灶性脑缺血再灌注期细胞死亡的作用

Insulin effect on death cells during reperfusion after ischemia in RHR

目的观察胰岛素对脑缺血再灌注后细胞死亡的影响，探讨胰岛素减轻脑缺血再灌注损伤的机制。方法利用肾血管性高血压大鼠，以线栓法复制ＭＣＡＯ，造成缺血２ｈ再灌注１、３、５ｄ，并于再灌注后立即使用胰岛素，采用ＴＵＮＥＬ法检测死亡细胞的变化。结果胰岛素治疗的各组死亡细胞数明显减少（Ｐ＜０．０５）。结论胰岛素减少脑缺血再灌注后细胞死亡是其减轻再灌注损伤的机制之一。

Objective To investigate the effect of insulin treatment on death cells of reperfusion injury Method The renovascular hypertension rats(RHRs) were done to MCAO as described by zea longa for 2 h By using the terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling DNA fragments in situ,the death cells or apoptotic cells were detected with TUNEL method at 1、3、5 days after reperfusion and insulin treatment Result The numbers of the death cells were significantly lower in the treated groups than control groups(P<0 05) Conclusion Insulin may reduce the death cells after reperfusion,which was probably one of the mechanisms that insulin reduces reperfusion injury