人类免疫缺陷病毒gag基因的单链构象多态性分析

Analysis on single-strand conformation polymorphism of human immunodeficiency virus(HIV-1)gag gene

艾滋病病例和人类免疫缺陷病毒感染者正在全球范围包括我国在内逐年上升，当进行基因疫苗研制时，首先应该搞清楚当地的流行毒株是什么。我们采用PCR-单链构象多态性分析技术，对主要来自云南地区的26份经确证的阳性血清标本进行了结构分析，发现其中15份的gag基因片段呈现一致的单链构象，与异源双链分析的结果基本一致，从而初步确定这类毒株为当地流行的主要毒株，为以后的序列测定及基因型确定奠定基础。

The number of patients with AIDS and HIV-1 infection is increasing rapidly around the whole world including our country. When the gene vaccines are developed the most important thing which should be thought first is what the prevailing isolates are in local region. We used PCR-single strand conformation polymorphism(SSCP) to make a structural analysis on gag gene of 26 HIV-1 isolates collected mainly from Yunnan province, southwest China. The results showed that 15 of them appeared the same features of SSCP on 8% PAGE which was paralleled with the results of Heteroduplex analysis. We concluded that these isolates were the prevalent ones in these regions. It laid a fundation for the sequencing and genetic typing further in the near future.