1991年、1996年淋病奈瑟菌耐药谱质粒谱相互关系研究

The relationship study between antibiotic-resistant profiles and plasmid profiles of Neisseria gonorrhoeae in 1991 and 1996 in Chengdu City

通过对成都地区1991年、1996年各50株淋病奈瑟菌的耐药谱质粒谱进行分析，以便探讨耐药谱与质粒谱之间相互关系。以琼脂扩散法测定每株标本对十二种抗生素的敏感性，碱裂解加溶菌酶法对其进行质粒抽提。结果表明：1991年分离的淋病奈瑟菌标本主要以同时耐2种抗生素为主，耐药谱型24种，质粒谱型5种。1996年分离的标本主要以同时耐3、4种抗生素为主，耐药谱型34种，质粒谱型增加到10种。提示：不同时期分离的标本耐药谱，质粒谱变化较大，同一时期分离的标本尽管耐药谱型较多，但质粒谱型相对稳定，说明本地区耐药菌株的流行存在染色体突变。耐药谱、质粒谱相互关系分析，为淋病奈瑟菌耐药性监测及准确及时地跟踪耐药菌株的来源和耐药菌株的流行特征分析提供了流行学信息。

Analysing the antibiotic-resistant profiles and plasmid profiles of 50 strains of N.gonorrhoeae in 1991 and 1996 respectively in Chengdu, in order to probe the relationship between antibiotic-resistant profiles and plasmid profiles of N. gonorrhoeae. Methods antibiotic susceptibility tests were performed by agarese diffusion and the plasmids were extracted hy alkaline lysis and enzymatic techniques. Results in 1991, most strains of N. gonorrhoeae were resistant to 2 kinds of antibiotic simultaneously. There were 24 types of antibiotic-resistant profiles, and 5 types of plasmid profiles. But, in 1996, the situation changed.most of strains were resistant to 3 or 4 kinds of antibiotic, and there were 34 types of antibiotic-resistant profiles and the plasmid profiles went up to 10 types. Conclusion the antibiotic-resistant profiles and plasmid profiles of N. gonorrhoeae isolated from different time have changed dramatically. The strains isolated during the same period though, there are many types of the antibiotic-resistant profiles the plasmid profiles are stable comparatively. It can he shown that the epidemic of resistant N. gonorrhoeae was probably caused hy the mutation of chromosome. The analysis of relationship between antibiotic-resistant profiles and plasmid profiles has provided the epidemiological information for monitoring the resistance of N. gonorrhoeae and tracing exactly and timely the sources of resistant strains and their epidemiological features.