慢性荨麻疹患者IL-4、IL-6及IFN-γ的检测

Detection of interleukin-4, interleukin-6 and interferon-γ in patients with chronic urticaria

为了了解T辅助细胞不同功能亚群在慢性荨麻疹发病机理中的作用，检测了46例慢性荨麻疹患者的外周血单个核细胞体外培养在PHA诱导后IL-4、IFN－γ以及IL-6的分泌，并以20例正常人作对照，结果发现患者的T辅助-2亚群（Th2）细胞因子IL-4的产生明显高于正常对照（P＜0．001），而T辅助-1亚群（Th1）的细胞因子IFN－γ却明显低于正常对照组（P＜0．05），细胞因子IL－6的产生在患者与正常人之间无明显的差别（P＞0．05）。此结果提示Th2细胞功能的表达增加可能在慢性荨麻疹的发病过程中起着重要的作用。

In order to assess the role of functional subsets of CD4+ T lymphocytes in chronic urticaria. 46 cases of chronic urticaria patients and 20 cases of normal individuals were selected in this study. The peripheral blood mononuclear cells (PBMC's) from chronic urticaria patients and normal volunteers were cultured in vitro with phytohemagglutinin (PHA) for 48 hours and the Th2 like cytokine IL-4, Th1 like cytokine interferon-γ(IFN-γ) and cytokine IL-6 level were measured in their cultured supernatant. The results showed that the PBMC's IL-4 secretion in chronic urticaria patients were significantly higher than that in normal controls(P＜0. 001), but the PBMC's IFN-γ secretion in chronic urticaria patients was markedly lower than in normal controls (P＜0. 05). The IL-6 lever showed no significantly difference between the patients and the normal controls (P＞0. 05). These data indicated that there are increased Th2 cell expression and decreased Th1 cell expression in chronic uriicatia patients and the increased expression of Th2 cells may have got an important role in the pathogenesis of chronic urticaria.