芭蕉芯及维生素B_6对小鼠肾草酸钙结晶抑制作用的研究

Morphometrical study on inhibitory effect of vitamin B 6 and banana stem extract on calcium crystalization

目的寻找预防及控制尿路结石的方法。方法在小鼠模型上用图像分析仪研究芭蕉芯提取液与维生素Ｂ６对肾草酸钙结晶形成的作用。将３６只小鼠分为正常对照组、成石组、芭蕉芯组和维生素Ｂ６组进行实验，３周后处死动物取肾脏分别进行结晶形态定量及钙和草酸测定。结果维生素Ｂ６组和芭蕉芯组小鼠肾脏结晶的面密度及数密度明显低于成石组；湿肾组织钙含量各组之间无显著差异，草酸含量在维生素Ｂ６和芭蕉芯组低于成石组。结论维生素Ｂ６和芭蕉芯提取液都具有抑制实验性高草酸尿症小鼠肾脏草酸钙结晶形成的作用。

Objective To search for the anticrystallization mechanism by vitamin B 6 and banana stem extract. Method Thirty six male China 1 mice were divided into 4 groups:normal, crystalization, vitamin B 6 and banana stem extract groups. Calcium oxalate crystalization was induced by 1% glycol and 1% ammonium chloride in the last 3 groups. Vitamin B 6 was then given to the 3rd group and banana stem extract to the 4th group for 3 weeks. At the end of 3 weeks, all the mice were sacrificed and the kidneys were taken for morphometrical studies using image analysis system and assayed for calcium oxalate. Result Morphometrical area density and numerical density of crystalization was significantly less in the vitamin B 6 group and the banana stem extract group as compared with the crystalization group, being the least in the banana stem extract group. Oxalate in renal tissue was also significantly reduced.Both vitamin B 6 and banana stem extract had no effect on renal tissue calcium. Conclusion Vitamin B 6 and banana stem extract may be useful agents in the treatment of patients with hyperocaluric urolithiasis.