肌酸激酶同工酶的提取纯化和重组

Purification and recombination of creatine kinase isoenzymes

目的对肌酸激酶（ＣＫ）同工酶进行纯化和重组。方法酶的制备和分子重组技术。结果纯化及重组后的ＣＫ－ＭＭ、ＣＫ－ＭＢ、ＣＲ－ＢＢ比活性分别为９３００００，６９２０００，４８１０００Ｕ·ｇ－１（３７℃）；ＳＤＳ－ＰＡＧＥ分析，ＣＫ－ＭＭ、ＣＫ－ＭＢ均呈现单带，而ＣＫ－ＢＢ为双带。

Objective To purify and recombine the creatine kinase(CK) isoenzyme. Methods\ Adopting purification and molecular recombinant techniques of enzyme. Results\ The specific activities of CK MM, MB and BB purified and recombined were 930 000 , 692 000 and 481 000 U·g -1 (37℃) respectively by SDS PAGE assay, both CK MM and MB showed single band, but CK BB showed double bands. Conclusions\ These results provide the basis for further investigation of preparation and immunoassay of CK MB monoclone antibody.