分泌血吸虫抗原杂交瘤的制备

Preparation of hybridoma expressing Schistosomiae antigen

目的制备分泌血吸虫抗原的杂交瘤。方法采用杂交瘤技术，将血吸虫成虫细胞与Ｓｐ２／０骨髓瘤细胞融合，ＨＡＴ选择性培养。结果获得了１株培养上清能与血吸虫病人血清起反应的杂交瘤，其染色体数为７２～８４，该杂交瘤细胞可持续培养１１周以上，但其生长缓慢，在培养８周后，出现细胞逐渐死亡。结论杂交瘤技术不适于远缘杂交。

Objective To preduce the hybridoma expressing Schistosomiae antigen. Method Cells from adult Schistosoma japonicum were fused with Sp 2/0 mouse myeloma cells, and cultured with HAT media. Results A positive clone was obtained, its chromosome number was 72￣84. The culture supernatant gave a positive reaction with serum from patients infected with S. japonicum, as demonstrated by ELISA. The hybridoma lived in HAT media over 11 weeks. After they were cultured for 8 weeks, the cells died slowly. Conclusion Cell death may be caused by segregation of DNA and loss of chromosome material. Hybridma technique is not suitable for distant hybridization.