妊高征患者胎盘中一氧化氮合酶-mRNA转录强度的变化及意义

The Changes and Significance of the Intensity of Transcription of Endothelial Nitric Oxide Synthase mRNA in Placental Tissue from Cases of Pregnancy Induced Hypertension

目的探讨妊高征患者胎盘中内皮型一氧化氮合酶（ｅＮＯＳ）含量减少的原因。方法采用定量的逆转录－聚合酶链反应（ＲＴＰＣＲ）技术，检测９例正常足月妊娠妇女胎盘和１２例妊高征患者胎盘中ｅＮＯＳｍＲＮＡ转录强度的变化。结果（１）正常足月妊娠妇女胎盘和妊高征患者胎盘中均存在ｅＮＯＳｍＲＮＡ。（２）妊高征患者胎盘中ｅＮＯＳｍＲＮＡ的转录强度显著低于正常足月妊娠妇女（Ｐ＜０．０５）。结论妊高征患者胎盘中ｅＮＯＳ含量的减少是由于ｅＮＯＳｍＲＮＡ转录强度下降所致。

Objective To determine the cause of decreasd expression of endothelial nitric oxide synthase (eNOS) in placental tissue from cases of pregnancy induced hypertension (PIH). Methods 9 placentae of normal pregnant women and 12 placentae of patients with PIH were studied. eNOS mRNA was measured in placental tissue by reverse transcription polymerase chain reaction (RT PCR). Results (1)There was eNOS mRNA in placental tissue of normal pregnancy as well as PIH cases. (2). The intensity of transcription of eNOS mRNA in placental tissue of PIH cases decreased significantly when compared with that of normal pregnancy ( P <0.05). Conclusion The decrease of the intensity of transcription of eNOS mRNA in placental tissue of PIH patients may result in reduction of expression of eNOS in placental tissue of PIH.