RP-HPLC法测定资木瓜中原儿茶酸的含量

Determination of Protocatechuic Acid in Chaenomeles lagenaria(Loisel) koidz by RP-HPLC

目的:建立以RP-HPLC法测定资木瓜中原儿茶酸含量的方法。方法:色谱柱:Agilent Eclipse XDB-C_(18)(250 mm×4.6mm,5μm),流动相:乙腈-0.1%磷酸水溶液(5:95),流速1 ml·min^(-1),检测波长260 nm。结果:原儿茶酸进样量在0.02～0.20μg范围内具有良好的线性关系(r=0.9999),平均加样回收率为101.23%,RSD为1.83%(n=5)。结论:本方法简便、准确、重复性好,可用于控制资木瓜的质量。

Objective： To establish a RP-HPLC method for the determination of protocatechuic acid in Chaenomeles lagenaria （Loisel） koidz. Method： The determination was performed on Agilent Eclipse XDB-C18 （250 mm × 4. 6 mm, 5 μm） column, with mo- bile phase consisted of acetonitrile-water（ containing 0.1% phosphotic acid） （5： 95 ）. The flow rate was 1.0 ml. min - 1 and the detection wavelength was 260 nm. Result： Protocatechuic acid showed a good linearity of 0. 02-0. 20μg （r =0. 999 9）. The average recovery was 101.23% with the RSD of 1.83% （n = 5 ）. Conclusion： The determination method is simple, accurate ,reliable, and can provide refer- ences for the quality control of Chaenomeles lagenaria（Loisel） koidz. K