小卫星DNA多态性用于脐血中母体细胞的检测

Application of minisatellite DNA hyperpolymorphism to detect maternal cells in cord blood

采用聚合酶链式反应（PCR）扩增具有高度多态性33．6位点，联合地高辛标记位点特异性寡核苷酸探针杂交，制作出PCR-小卫星DNA片段长度多态性图谱，对我院常规法收集的脐带血30份（1份用于脐带血移植）进行了母体细胞的检测。结果显示，小卫星DNA多态性是特异的遗传标志物，30份被检脐血均未测到母体细胞标志物。该法特异、灵敏、简便、快速，无同位素污染，可作为脐血质量监控的可靠方法。

The maternal cells in cord blood were detected by PCR-restriction fragment length polymorphism pettern of minisatellite DNA combined with digoxigenin-labeled locus-specific oligonucleotide hybridization- Minisatellite DNA polymorphism by this technique was used as a specific gentic marker to detect the maternal cells in of cord blood 30 cases. The results showed that this marker is genetically stable and individual1y specific. All the samples of cord blood were negative for maternal cells. The technique is specific, sensitive, rapid and has no contamination from radiosotopes, therefore it is a usefull method for monitoring quanlity of cord blood by cord blood bank for transplantation.