摘要
目的：建立ＩＬ－１０基因疗法的实验模型，并动态观察其体内ＩＬ－１０分泌水平。方法和结果：构建携带鼠ＩＬ－１０基因的重组逆转录病毒载体，体外转染小鼠ＮＩＨ３Ｔ３成纤维细胞，筛选到１株高分泌ＩＬ－１０的细胞克隆株；将此阳性克隆株移植到小鼠腹腔内，可在小鼠血清中检测出ＩＬ－１０的活性。
Objective: To establish an experimental model of IL 10 gene therapy with fibroblasts. Methods and results: By infecting the murine fibroblast cell line NIH3T3 with pLGSN retrovirion,a cell clone designated as NIH3T3 IL 10 secreting the highest IL 10 level was obtained. The cell clone was expanded in vitro, encapsulated into collagen and implanted i.p. into mice. The activity of IL 10 could be detected in mouse serum. Conclusion: These results demonstrate that fibroblasts could successfully transfer the IL 10 gene which could express in vivo.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1998年第6期533-535,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
