人类巨细胞病毒感染及肾移植排斥后HLA-DR抗原的表达

The expression of HLA DR antigen on T lymphocytes following cytomegalovirus infection and graft rejection episode in renal recipients

目的：探讨ＨＬＡＤＲ抗原在人类巨细胞病毒（ＨＣＭＶ）感染和肾移植排斥患者外周血Ｔ淋巴细胞上的表达及临床意义。方法：用流式细胞仪检测肾功能正常１０例、慢性排斥而无ＣＭＶ感染（血肌酐２００～５００μｍｏｌ／Ｌ）８例、ＣＭＶ活动性感染６例、对照组６例４组的ＨＬＡＤＲ抗原的表达。结果：排斥组和活动性感染组ＨＬＡＤＲＣＤ８＋细胞表达明显增高，４组ＣＤ４＋细胞的表达无显著差异，且ＨＣＭＶ感染组ＣＤ４＋／ＣＤ８＋比率倒置。结论：ＣＤ８＋在抗病毒免疫中起重要作用；ＨＣＭＶ亦可引起Ｔ淋巴细胞激活，参与免疫反应，诱发排斥反应的发生。

Objective:To inquire into The expression of HLA DR antigen on T lymphocytes following cytomegalovirus infection and graft rejection episode. Methods:Flowcytometry was applied to detect the expression of HLA DR in 10 stable graft function, 8 chronic rejection, 6 HCMV active infection and 6 controls. Result: No significant differences in HLA DR expression were observed in the CD4+ reactive subpopulation among these controls and patients. The expressions of HLA DR on CD8+ were higher in active human cytomegalovirus infection and rejection patients than that in control groups and stable graft function patients, Active HCMV infection was associated with an inverted ratio of CD4+/CD8+.When infection or rejection were controlled, the expression of HLA DR was often irreversible. Conclusion:CD8+ may play an important role in the course of antivirus immunity,Active HCMV infection was associated with allograft rejection and infection of tentimes induces allograft rejection.