乙型肝炎后肝硬化患者外周血IL-2、IFN-γ活性与肝功能分级的关系

Ralationship between peripheral blood IL 2,IFN γ activities and Child Pugh classification

目的：探讨乙型肝炎后肝硬化（ＰＨＣ）患者外周血白介素２（ＩＬ２）和γ干扰素（ＩＦＮγ）活性与肝功能状态的关系。方法：测定５６例ＰＨＣ患者和２４例健康成人外周血淋巴细胞ＩＬ２受体（ＩＬ２Ｒ）的表达，ＩＬ２和ＩＦＮγ生成及血清可溶性ＩＬ２受体（ｓＩＬ２Ｒ）。结果：ＰＨＣ组和对照组的ＩＬ２活性与ＩＬ２Ｒ表达率、ＩＦＮγ活性之间均呈正相关，与ｓＩＬ２Ｒ水平则负相关。与对照组比较，ＰＨＣ组ＩＬ２Ｒ表达，ＩＬ２和ＩＦＮγ生成均显著低下（Ｐ＜０．０５、＜０．０１、＜０．００１），而ｓＩＬ２Ｒ水平则显著升高（Ｐ＜０．００１）；而且随着ＣｈｉｌｄＰｕｇｈ肝功能分级之降低，上述这种改变亦明显。结论：ＰＨＣ患者存在一定程度的Ｔ细胞功能障碍与缺陷。

Objective:To explore the correlation between the liver function status and the activities of interleukin 2(IL 2)and gamma interferon(IFN γ) in patients with hepatitis B cirrhosis (PHC).Methods:In 56 patients with PHC and in 24 heathy adults as control,the production of IL 2 and IFN γ and the expression of IL 2 receptor (IL 2R)on peripheral blood lymphocytes (PBL) induced by PHA P,the serum level of soluble interleukin 2 receptor (sIL 2R) was measured.Results:There was a significant linear correlation between the production of IL 2 and IFN γ and the expression of IL 2R(P<0.001 and P<0.01,respectively), and a significant reverse correlation between the serum level of sIL 2R and the activity of IL 2 as well as the expression of IL 2R(P<0.01,<0.05) respectively.The expression of IL 2R and the production of IL 2 and IFN γ in PHC's patients were markedly lower than those of the control group(P<0 05,<0.001 and <0.001),respectively,but the serum level of sIL 2R was significantly higher than that in the control group (P<0.001).Compare these with Child Pugh's classification of cirrhosis ,we found the poorer the liver function,the lower the expression of IL 2R,the production of IL 2 and IFN γ,and the higher the sIL 2R level.Conclusions:The immune function of T cells in patients with PHC decrease and are closely correlated with their liver function status.