静脉输注脂肪乳剂对急性坏死性胰腺炎大鼠脂质炎性介质的影响

Effects of intravenous fat emulsion on lipid mediators during acute necrotizing pancreatitis in rats

目的：研究静脉输注脂肪乳剂对大鼠急性坏死性胰腺炎早期脂质介质的影响。方法：逆行胰管注射３％牛磺胆酸钠建立ＳＤ大鼠坏死性胰腺炎动物模型，通过颈内静脉营养支持７２小时，测定４、４８、７２小时血清淀粉酶、ＴＸＢ２、６ｋｅｔｏＰＧＦ１α、ＰＧＥ２，７２小时行胰腺组织病理学评分。结果：４小时胰腺炎组大鼠血前列腺素类物质均增高，应用脂肪乳剂后可增加血６ｋｅｔｏＰＧＦ１α（糖脂组１５６９±１１７３ｐｍｏｌ／Ｌ；全糖组２９１±６１ｐｍｏｌ／Ｌ，Ｐ＜００５）、ＰＧＥ２（脂肪组６１４±１５ｐｍｏｌ／Ｌ；全糖组２８７±９ｐｍｏｌ／Ｌ，Ｐ＜００５）含量，病理学评分全糖组和糖脂组间无差异。

Objective: To study the effects of intravenous fat emulsion on lipid mediators during early stage of acute necrotizing pancreatitis (ANP) in SD rats. Methods: ANP was induced by retrograd pancrcatic duct infusion of 3% taurocholate sodium. All animals received parenteral nutrition through the central vein for 72hrs. Serum Amylase (PNPG 7),6 keto PGF 1α , TXB 2, PGE 2were determined by RIA at 4, 48, 72h; The pancreas was dissected for histological scoring at 72h. Results: prostaglandins were increased in ANP groups at 4h. Intravenous fat emulsion could increase serurn levels of 6 keto PGF 1α , (156 9±117 3pmol/L; 29 1±6 1pmol/L, in fat and glucose administrating groups respectively, P<0 05, PGE 2(61 4±15 0pmol/L; 28 7±9pmol/L, and in fat and glucose groups respectively) at 4h, Histological scoring showed no defference between glucose group and fat group. Conclusion: Fat emulsion infused in the early stage of ANP can induce increase of serum anti inflammation prostaglandins.