中药“425”合剂诱导的抗血吸虫卵肉芽肿效应及其机制研究

Effects and Mechanisms of Anti-S.japonicum Egg Granuloma Induced by Chinese Materia Medica "425" Combining Reagent

改“425”制剂（中药当归提取物）为口服型，并伍用鲨鱼软骨粉（SCP），制成“425”合剂，用以研究感染血吸虫3周的BALB／c小鼠服用“425”合剂后4周诱导的抗肝内虫卵肉芽肿（SEG）效应及机制。测定小鼠肝内SEG大小，用EtnA检测小鼠血清抗体IgG及其亚类IgGI水平（OD均值）；采用流式细胞仪测定小鼠脾脏T细胞亚群的细胞均数和比值。服用“425”合剂（组Ⅰ）和单用SCP（组Ⅲ）小鼠的SEG大小显著小于感染对照鼠（组Ⅳ），组Ⅰ、组Ⅱ（单用“425”制剂）和组Ⅲ的SEG减小车之间无显著性差异；组Ⅰ和组Ⅱ的IgG和ISG1水平均显著高于组Ⅲ和组Ⅳ者；组Ⅰ和组Ⅲ的CD4+／CD8+比值（1．70－1．88）明显高于组Ⅱ（0．95）。实验结果表明，“425”合剂能诱导明显的抗肉芽肿效应，其机制与体液免疫和细胞免疫密切相关；SCP能非常显著诱导细胞免疫效应，特别是具有抗肝内纤维化作用；“425”制剂能明显诱导体液免疫效应。‘本项研究尚未见有类似报道。

To investigate the effects and mechanisms of anti - granuloma formation induced by '425' conlbining reagent (combination of shark cartilage powder with '425' reagent ). The mean sizes of schistosome egg granuloma (SEG) were measured.The specific antibody IgG and its subclass IgG, levels, and the percentage of splenic Iymphocyte phenotypes of BALB/cmice 4 weeks (7 weeks post - infection)afler orally adminislering the '425' combining reagent (group I ) . '425' reagenlonly(group Ⅱ )and shark cartilage powder(SCP) only (group Ⅲ ) respectively were detected. The mean sizes of SEG ingr(iup Ⅰ and group Ⅲ were significantly decreased than those in group Ⅳ (infeclion control)and the hepatic fibro5is in bothgroups obviously disappeared. The reduchon rates of SEG had no differences markedly among group Ⅰ to Ⅲ. The IgG andIgG, of group Ⅰ and group Ⅱ were both significantly higher than those in group Ⅲ and group Ⅳ. The ratios of CD4 + /CD8 + in group Ⅰ and group Ⅲ (1 .70 - 1. 88) were obviously higher than those in group Ⅱ (0. 95 ). '425' combiningreagent could induced obviously anti - granuloma effects and its mechanisms were associaled with humoral and cellular immune responses. SCP could induced very strong cellular immune responses and had especially effects on anti - hepatic fibrosis. '425' reagent(angelica root extract)could induced major humoral immune responses. The research improved basictheory and will contribute to practice in the future. No similar reports have beenfound up to now.