腺苷对大鼠缰核神经元自发放电活动及c-fos蛋白表达的影响

The effects of adenosine on the discharging electricities and the c-fos expression of neurons in habenula nucleus of rats

目的:观察腺苷(Adenosine,Ado)对缰核(Habenula nucleus,Hb)神经元单位放电的影响及外侧缰核内c-fos蛋白表达的变化,探讨腺苷对影响睡眠的缰核神经元活动及相关基因表达的影响及可能机制。方法:脑薄片灌注、腹腔内注射、原位免疫细胞化学等。结果:脑薄片灌注腺苷,导致内侧缰核神经元自发放电活动受到抑制,而外侧缰核神经元自发放电活动明显增加;腹腔注射腺苷0.5小时后,与注射生理盐水组相比较,外侧缰核c-fos蛋白的表达量明显增加。结论:腺苷对大鼠内侧缰核神经元自发放电起抑制作用,对外侧僵核神经元自发放电则有兴奋作用,并可促进外侧缰核c-fos蛋白表达,这为腺苷在外侧缰核内可促进睡眠提供了依据。

Objective：To investigate the effect of adenosine （Ado） on the unit discharging electricities in habenula nucleus and on the c-fos expression in lateral habenular complex, and the influence of adenosine on the neuron activities and related gene expression involved in affecting sleep in habenula nucleus and the possible mechanisms. Methods： Intraperitoneal injection, brain flakes pouring of rats, immunohistochemistry and other methods were useel. Results： Ado pouring into flakes of brain depressed the unit discharging electricities of neurons in medial habenular complex（MHb）, but obviously increased that of lateral habenular complex （LHb）. 0.5 h after the six rats being injected intraperitoneally with Ado, the c-fos protein expression in lateral habenular nucleus was significantly increased compared to the group with saline injection. Conclusion： Ado may restrain the unit discharging electricities of neurons in medial habenular complex but excite those in lateral habenular complex. At the meantime,Ado may increase the c-los expression in LHb. This provides the experimental evidence that Ado may improve the sleep quality.