Toll样受体对睾丸Sertoli细胞免疫调节的初步研究

The preliminary research of relationship between Toll-like receptor and the immunoloregulation of Sertoli cell

目的:研究睾丸Sertoli细胞感染后,TLR发挥免疫调节功能的情况。方法:正常大鼠Sertoli细胞表达Toll样受体的情况以及用溶脲脲原体(Ureaplasma Urealyticum,UU)体外感染Sertoli细胞后,分别在12、24、36小时时间段比较感染组与对照组之间Toll样受体表达变化情况。结果:正常大鼠Sertoli细胞表达TLR2-8,低表达TLR9和10,未检出TLR1和5;与对照组相比,体外感染处理后TLR2、6表达增加。结论:Toll样受体的激活参与Sertoli细胞对炎症的免疫调节,两者具有一定的联系。

Objective：To investigate the relationship between Toll-like receptor and the immune regulation about inllawanation by Sertoli cell in vitro. Methods：Here we examined the expression and potential functions of TLR family in rat Sertoli cells. Using our well-characterized urealyticum（UU） induced model we tested the expression changes of TLR2 and TLR6 at12th,24th,36th hours after UU infection in vitro. Results：We demonstrated that TLR2-8 are highly expressed;TLR9 and TLR10 are expressed at relatively low level;the expression of TLR1 and TLR5 are not detected in normal rat Sertoli cells. Comparing with coutrol group, Sertoli cells express more TLR2 and TLR6 after infected by UU. Conclusion： There is some relationship between the activation of TLRs and the immune regulation about inflammation by Sertoli cell.