黄冠梨茎尖离体培养再生体系建立初探

Primary study of regeneration system of pear cultivar 'Huangguan' stem apex by in vitro culture

为建立黄冠梨离体培养高效再生体系,以茎尖为外植体,设计不同消毒处理,外植体离体培养30d后,获得初代培养无菌苗,再通过正交试验设计对增殖和生根适宜培养基进行筛选。结果表明,茎尖消毒以75%酒精处理10s、再用0.1%HgCl2浸泡4min的消毒效果最佳,适宜增殖培养基为AS+6-BA0.8mg/L+NAA0.1mg/L,适宜生根培养基为1/2MS+IBA1.0mg/L。

In order to establish the high efficient regeneration system of pear cuhivar ＇Huangguan＇ by in vitro culture, using the stem apex as material, the different treatments for sterilization of stem apex were designed to study optimal sterilization schedule. The plantlets was gotten after 30 days initial culture. The suitable medium for budding and rooting was screened out by orthogonal test design. The results showed that the optimal sterilization schedule was treatment with 75% ethanol for 10 seconds, then with 0.1%HgCl2 for 4 minutes. The suitable budding medium was AS＋6-BA0.8 mg/L＋NAA0.1 mg/L and the suitable rooting medium was 1/2MS＋IBA1.0 mg/L.