菟箭合剂含药血清对高糖条件培养的肾小球系膜细胞磷酸化Akt和PTEN的作用

Seropharmacological Effect of Tujian Mixture on Phospho-Akt and PTEN of Glomerular Mesangial Cell Cultured in High Concentrations of Glucose

目的观察中药菟箭合剂含药血清对高糖条件培养的大鼠系膜细胞(MC)磷酸化Akt和PTEN的作用。方法将大鼠肾小球系膜细胞分为正常对照组、高糖条件培养组和高糖条件培养+4个不同浓度菟箭合剂含药血清组,不同条件培养液培养72h后用双夹心ELISA法检测细胞内磷酸化Akt(Thr308)和磷酸化PTEN(Ser380)含量。结果高糖组MC磷酸化Akt(Thr308)水平显著高于正常对照组(P<0.01),菟箭合剂含药血清干预的MC磷酸化Akt水平显著低于高糖组(P<0.01),且有明显的量效关系。高糖组MC磷酸化PTEN(Ser380)水平显著低于正常组MC(P<0.01),各种浓度菟箭合剂含药血清组PTEN水平均高于高糖组,且呈现量效关系。结论中药菟箭合剂具有提高高糖培养的MC细胞内PTEN水平,抑制Akt激活的作用。

Objective To observe the effects of serum containing the medicine of Tujian Mixture on phospho-Akt and phospho-PTEN of glomerular mesangial cell （MC） cultured in high concentrations of glucose. Method The glomerular mesangiai cells from SD rat were divided into six groups： the normal control group, the group of MC cultured in high concentrations of glucose, and four other groups cultured in high concentrations of glucose ＋ different concentrations of rat＇s serum containing Tujian Mixture. After 72 hours, the content level of phospho-Akt （Thr308） and phospho-PTEN （Ser380） in MC was detected by using sandwich ELISA. Results The level of phospho-Akt （Thr308） in the group of MC cultured in high concentrations of glucose was significant higher than that in the normal control group （P 〈0.01）. The level of phospho-Akt （Thr308） in the groups with serum containing Tujian Mixture was significant lower than that in the group of MC cultured in high concentrations of glucose （P〈0.01）. The effects indicated a remarkable change with different dosage of Tujian Mixture. Furthermore, the level of phosphated PTEN （Ser380） in the group of MC cultured in high concentrations of glucose was significant lower than that in the normal control group （P〈0.01）. The level of phosphated PTEN in the groups with serum containing Tujian Mixture was significant higher than that in the group of MC cultured in high concentrations of glucose （P〈0.01）. The effects also indicated a relationship with the dosage of Tujian Mixture. Conclusion Chinese herbal medicine Tujian Mixture can increase the level of phosphated PTEN and inhibit activation of Akt in cultured MC in high concentrations of glucose.