安神健脑液质量标准研究

Study on the Quality Standard of Anshen Jiannao Mixture

目的建立安神健脑液的质量标准。方法采用薄层色谱法鉴别麦冬、丹参、枸杞子和人参;采用高效液相色谱法测定五味子中五味子醇甲的含量,C18(250mm×4.6mm,5μm)色谱柱,流动相为甲醇-水(55∶45),流速1.0mL/min,检测波长250nm。结果在薄层色谱中可检出丹参、枸杞子和人参的特征斑点;五味子醇甲在0.1218～1.218μg范围内线性关系良好,r=0.9999,精密度RSD=0.22%(n=6),重复性试验RSD=1.1%(n=6),平均加样回收率为97.34%(RSD=1.7%,n=6)。结论本方法简便、灵敏、专属性强、重复性好,可作为安神健脑液的质量控制方法。

Objective To study the quality standard of Anshen diannao Mixture. Methods Radix ophiopgonis, Radix et Rhizoma Salviae Mfltiorrhizae, Fructus Lycii and Radix et Rhizoma Ginseng were identified by TLC. HPLC was used to determine the content of Schizandrol A on C18 （250 mm×4.6 mm, 5 μm） ODS column. The mobile phase was consisted of methanol-water （55 ： 45）. The flow rate was 1.0 mL/min. The detection wavelength was at 250 nm. Results The characteristic spot of Radix et Rhizoma Salviae Miltiorrhizae, Fructus Lycii and Radix et Rhizoma Ginseng could be easily examined by TIC. The calibration curve of Schizandrol A was linear in the range of 0.121 8～ 1.218 μg, r =0.999 9. The precision RSD was 0.22% （n =6）. The reproducibility RSD was 1.1% （n=6）. The average recovery rate of Sehizandrol A was 97.34% （RSD=1.7%, n=6）. Conclusions The method is easy, sensitive and specific with good reproducibility. It can be used to control the quality of Anshen diannao Mixture.