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转录因子AP-2β与SLP-2的蛋白相互作用 被引量:1

The Interaction Between Transcription Factor AP-2β and SLP-2
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摘要 AP-2β是转录因子AP-2家族中的重要一员.AP-2是一个与哺乳动物的发育、细胞生长、分化、凋亡和肿瘤发生都有密切联系的重要的转录因子家族.最近的研究表明,AP-2能通过与其他蛋白的相互作用来调控AP-2下游基因的转录活性.采用免疫共沉淀结合质谱鉴定的方法筛选与AP-2β相互作用的蛋白.结果筛选到Stomatin like protein2(SLP-2)蛋白可能与AP-2β相互作用.免疫共沉淀实验证实外源过表达和内源的SLP-2蛋白都能与AP-2β蛋白相互作用.而且,细胞免疫荧光实验发现外源表达的Myc-AP-2β蛋白与内源的SLP-2蛋白在MCF-7细胞的胞质中有共定位.此外,免疫印记实验结果表明过表达SLP-2能上调AP-2β的蛋白水平,这就表明SLP-2与AP-2β相互作用后,SLP-2可能使AP-2β蛋白更稳定.这些研究结果为进一步研究这两个基因的功能提供了新的切入点. AP-2β is an important member of transcription factor AP-2 family. AP-2 is a transcription factor implicated in mammalian development, cell proliferation, apoptosis and carcinogenesis. Recent studies have shown that interaction partners can modulate the transcriptional activity of AP-2 over the downstream targets. To identify potential AP-2β interacting partners, we screened AP-2β immunocomplexes by mass spectrometry and identified SLP-2 as a putative AP-2β interacting protein. It is demonstrated that both exogenously expressed and endogenous SLP-2 interacted with AP-2βin vivo by co-immunoprecipitation assays. In addition, indirect immunofluorescent analyses demonstrated that AP-2β and SLP-2 co-localized in the cytoplasm of the MCF-7 cell lines. Furthermore, over-expression of SLP-2 enhanced the protein expression level of AP-2β. Thus, through protein-protein interaction, SLP-2 can stabilize AP-2β. These results provided a new clue for further research on the AP-2β and SLP-2 function.
出处 《生命科学研究》 CAS CSCD 2010年第1期16-20,共5页 Life Science Research
基金 国家973资助项目(2005CB522505) 国家973资助项目(2006CB943506) 湖南省科技厅资助项目(2007CK3052) 湖南省教育厅资助项目(07c563)
关键词 AP-2β 相互作用蛋白 SLP-2 免疫荧光分析 免疫共沉淀 AP-2β interacting protein SLP-2 immunofluorescent analysis co-immunoprecipitation
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参考文献15

  • 1WILLIAMS T, TJIAN R. Characterization of a dimerization motif in AP-2 and its function in heterologous DNA-binding proteins[J]. Science, 1991, 251: 1067-1071.
  • 2WILLIAMS T, TJIAN R. Analysis of the DNA-binding and activation properties of the human transcription factor AP-2[J]. Genes Dev, 1991, 5: 670-682.
  • 3ZHANG J, HAGOPIAN-DONALDSON S, SERBEDZIJA G, et al. Neural tube, skeletal and body wall defects in mice lacking transcription factor AP-2[J]. Nature, 1996, 381 (6579) : 238-241.
  • 4SCHORLE H, MEIER P, BUCHERT M, et al. Transcription factor AP-2 essential for Cranial closure and craniofacial development[J]. Nature, 1996, 381: 235-238.
  • 5MOSER M, PSCHER A, ROTH C, et al. Enhanced apoptotic cell death of renal epithelial cells in mice lacking transcription factor AP-2beta[J]. Genes Dev, 1997, 11: 1938-1948.
  • 6PELLIKAINEN M J, PEKOLA T T, ROPPONEN K M, et al. p21^WAF1 expression in invasive breast cancer and its association with p53, AP-2, cell proliferation, and prognosis[J]. Clin Pathol, 2003, 56(3): 214-220.
  • 7MCPHERSON L A, WEIGEL R J. AP-2alpha and AP- 2gamma: a comparison of binding site specificity and transactivation of the estrogen receptor promoter and single site promoter constructs[J]. Nucleic Acids Res, 1999, 27(20): 4040-4049.
  • 8PERISSI V, MENINI N, COTTINE E, et al. AP-2 transcription factors in the regulation of ERBB2 gene transcription by oestrogen[J]. Oncogene, 2000, 19(2): 280-288.
  • 9WANG Y, MORROW J S. Identification and characterization of humanSLP-2, a novel homologue of stomatin (band7.2b) present in erythrocytes and other tissues[J]. J Biol Chem, 2000, 275 (11): 80622-80631.
  • 10SEDENSKY M M, SIEFKER J M, KOH J Y, et al. A stomatin and a degenerin interact in lipid rafts of the nervous system of Caenorhabditiselegans[J]. Am J Physiol Cell Physiol, 2004, 287(2): C468-C474.

二级参考文献10

  • 1Comis RL. A brief history of the research and treatment of lung cancer from 1970 to 2000[J]. Int J Clin Oncol, 2003, 8(4):230-233.
  • 2Curiel DT, Gerritsen Wr, Krul MRL. Progress in cancer gene therapy[J]. Cancer Gene Therapy, 2000,7(8):1197-1199.
  • 3Haura EB, Sotomayor E, Antonia SJ. Gene therapy for lung cancer[J]. Mol Biotechnol, 2003, 25(2):139-148.
  • 4Kawai H, Kiura K, Tabata M, et al. Characterization of nonsmall-cell lung cancer cell lines established before and after chemotherapy[J]. Lung Cancer, 2002, 35(3):305-314.
  • 5Baas P. Inductive and adjuvant treatment strategies for localized non-small cell lung cancer in operable and inoperable patients [J].Curr Opin Oncol, 2002, 14(2):180-184.
  • 6Quest AF, Leyton L, Parraga M. Caveolins, caveolae, and lipid rafts in cellular transport, signaling, and disease [J]. Biochem Cell Biol, 2004, 82(1):129-144.
  • 7Wang Y, Morrow JS. Identification and characterization of human SLP-2, a novel homologue of stomatin (band 7.2b) present in erythrocytes and other tissues [J]. J Biol Chem, 2000, 275(11):8062-8071.
  • 8Owczarek CM, Treuflein HR, Portbury KJ, et al. A novel member of the STOMATIN/EPB72/mec-2 family, stomatin-like 2 (STOML2), is ubiquitously expressed and localizes to HSA chromosome 9p13.1[J]. Cytogenet Cell Genet, 2001, 92(3-4):196-203.
  • 9Chelur DS, Ernstrom GG, Goodman MB, et al. The mechanosensory protein MEC-6 is a subunit of the C. elegans touch-cell degenerin channel [J]. Nature, 2002, 420(6916):669-673.
  • 10You Z, Gao X, Ho MM, et al. A stomatin-like protein encoded by the slp gene of Rhizobium edi is required for nodulation competitiveness on the common bean[J]. Microbiology, 1998, 144(Pt 9):2619-2627.

共引文献10

同被引文献19

  • 1Zarelli VE, Dawid IB. Inhibition of neural crest formation by Kctdl5involves regulation of transcription factor AP-2 [ J ]. Proc Natl AcadSci USA, 2013,110(8) : 2870-2875.
  • 2Praetorius C,Grill C, Stacey SN, et al. A polymorphism in IRF4affects human pigmentation through a tyrosinase-dependent MITF/TFAP2A pathway[J]. Cell, 2013, 155(5) : 1022-1033.
  • 3Wenke AK, Bosserhoff AK. Roles of AP-2 transcription factors in theregulation of cartilage and skeletal development [ J] . FEBS J, 2010,277(4) : 894-902.
  • 4Handwerger S. New insights into the regulation of human cytotropho-blast cell differentiation[ J], Mol Cell Endocrinol,2010,323 ( 1);94-104.
  • 5Zarelli VE, Dawid IB. Inhibition of neural crest formation by Kctdl5involves regulation of transcription factor AP-2 [ J]. Proc Natl AcadSci USA, 2013, 110(8) : 2870-2875.
  • 6Carrifere C,Mirocha S, Deharvengt S, et al. Aberrant expressions ofAP-2a splice variants in pancreatic cancer [ J ] . Pancreas, 2011 , 40(5) : 695-700.
  • 7Chen L, Zhu H, Pan Y, et al. Ascorbic acid uptaken by sodium-dependent vitamin C transporter 2 induces phCG expression throughSpl and TFAP2A transcription factors in human choriocarcinoma cells[J]. J Clin Endocrinol Metab, 2012,97(9) : E1667-1676.
  • 8Nakaya Y, Shimode S, Kobayashi T, et al. Binding of transcriptionfactor activating protein 2 7 on the Sproximal promoter region ofhuman porcine endogenous retrovirus subgroup A receptor 2/GPR172B[J]. Xenotransplantation, 2012, 19(3) : 177-185.
  • 9Zhang X,Leung YK, Ho SM. AP-2 regulates the transcription ofestrogen receptor ( ER) by acting through a methylation hotspot ofthe ON promoter in prostate cancer cells [ J ]. Oncogene, 2007,26(52) : 7346-7354.
  • 10Qin HR, Iliopoulos D, Nakamura T, et aJ. Wwox suppresses pros-tate cancer cell growth through modulation of ErbB2 -mediated andro-gen receptor signaling [ J ]. Mol Cancer Res,2007 , 5(9): 957-965.

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