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外源性VEGF对结肠癌细胞SW480蛋白质变化的影响

Protein Changes in Colon Cancer Cells After VEGF Stimulation Using Proteomic Technique
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摘要 利用RT-PCR发现结肠癌细胞存在血管内皮生长因子(VEGF)及其受体(VEGFR)的表达,随后采用外源性VEGF对结肠癌细胞SW480进行体外刺激,流式细胞仪检测到结肠癌细胞刺激后快速进入分裂期,同时提取VEGF作用前后的结肠癌细胞总蛋白,蛋白质组学研究,发现存在明显的蛋白质变化,其中鉴定出10个差异蛋白质.提示外源性VEGF可以促进结肠癌细胞增殖并对肿瘤细胞蛋白质组产生明显的影响. VEGF and VEGFR cancer cells were treated with expressed in colon cancer cells exogenous VEGF and we found detected by RT-PCR. Then, the colon that VEGF could promote cancer cells disintegrate detected by flow cytometry. Many protein spots separated by two-dimensional gel electrophoresis changed after VEGF stimulated and 10 proteins were identified by peptide mass fingerprinting. It indicated that colon cancer protein changes obviously after VEGF stimulated.
作者 王学虎 傅仲学 赵渝 谌伟 吴星烨
机构地区 重庆医科大学附属第一医院普通外科
出处 《生命科学研究》 CAS CSCD 2010年第1期62-66,共5页 Life Science Research
基金 国家自然科学基金资助项目(30772547)
关键词 血管内皮生长因子 蛋白质组 双向凝胶电泳 结肠癌 vascular endothelial growth factor proteome two-dimensional gel electrophoresis colon cancer
分类号 R735.35 [医药卫生—肿瘤]
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参考文献9

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生命科学研究
2010年 第1期

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