凉血活血胶囊含药血清对微血管异常生成的影响

Effect of Serum Containing Components of Liang Xue Huo Xue Capsules on the Angiogenesis

目的观察临床治疗银屑病的经验方凉血活血胶囊含药血清对微血管异常生成的作用,探讨其治疗银屑病的作用机制。方法以鸡胚绒毛尿囊膜(chorioallantoic membrane,CAM)为模型观察凉血活血胶囊含药血清对血管生成的影响;采用碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)及12-十四酸佛波酯-13-乙酸盐(phorbol-12-myristate-13-acetate,PMA)诱导的人真皮微血管内皮细胞株(human dermal microvascular endothelial cells,HDMEC)异常增生模型,以cck-8比色法测定凉血活血胶囊含药血清对其增生的影响;采用transwell细胞迁移、体外管腔形成方法观察凉血活血胶囊含药血清对HDMEC迁移及管腔形成能力的影响。结果与相同浓度的空白血清组相比,5%、10%、15%的凉血活血胶囊含药血清均在不同程度上抑制CAM血管新生(分别P<0.05,P<0.01);对HDMEC增生也表现出一定的抑制作用(分别P<0.05,P<0.01);均可抑制HDMEC的迁移(P<0.01);并可显著抑制HDMEC的管腔形成(P<0.01)。结论凉血活血胶囊含药血清可抑制CAM血管新生、对异常HDMEC的增生、迁移、微血管管腔形成均有抑制作用,提示凉血活血胶囊作用于血管异常生成环节,可能是临床治疗银屑病的作用机制之一。

Objective This study aimed to explore the possible effect on angiogenesis of Liang Xue Huo Xue（LXHX） Capsules, derived from a clinical effective decoction and to understand the mechanism in treating psoriasis. Methods The chorioallantoic membrane（CAM） model was established to investigate the effect of drug serum with LXHX Capsules on the angiogenesis. Abnormal hyperplasia models of human dermal microvascular endothelial cells（HDMEC） stimulated by basic fibroblast growth factor（bFGF） and phorbol-12-myristate-13-acetate（PMA） were used to survey HDMEC proliferation by the cck-8 assay, cells migration by Transwell assay and lumen formation by lumenforming assay. Results As compared with the same serum concentration of serum that did not contain LXHX, the 5%,10% and 15% serum containing LXHX capsule components significantly inhibited angiogenesis of the CAM（P〈0.05, P〈0.01, respectively） and HDMEC proliferation（P〈0.05, P〈0.01, respectively）, as well as significantly inhibited HDMEC migration（P〈0.01） and lumenformation（P〈0.01）. Conclusion The contained LXHX capsules components inhabited CAM angiogenesis, HDMEC proliferation, migration and lumenformation which indicated that LXHX capsules affect on angiogenesis and it may be one of the mechanisms of treating psoriasis with LXHX capsule.