胚胎神经干细胞对脑损伤治疗作用的实验研究

Experimental research on the curing effect of neural stem cells on brain injury

目的近年来,脑损伤的发病率呈不断上升的趋势,而有关脑损伤后的修复机制尚不完全清楚。本研究旨在探讨胚胎神经干细胞(NSCs)在脑损伤修复过程中的作用,为临床利用胚胎NSCs治疗脑损伤提供理论依据。方法通过冷冻伤的方法建立小鼠颅脑损伤模型,体外分离、培养和纯化E14d的绿色荧光蛋白(GFP)转基因小鼠胚胎神经干细胞,对NSCs进行诱导分化,通过眶后静脉丛将NSCs移植到脑损伤模型鼠体内,通过转轮式疲劳实验等多种方法进行神经功能评估,并通过形态学方法检测移植的NSCs在鼠脑组织的生长及分布情况。结果采用脑冷冻伤模型,致小鼠脑损伤导致了小鼠行为学和病理学改变。体外实验证明分离、培养并纯化的NSCs具有自我增殖能力,并且可以分化为神经元和星形胶质细胞。移植后的NSCs不仅可以与小鼠脑组织较好的整合,并可定向迁移到损伤部位,替代损伤脑组织。结论外源性NSCs可以在脑损伤小鼠脑内存活,并能促进脑损伤小鼠的神经功能恢复。

Objective To study the curing effect of NSCs on TBI recovery course,to provide available information for clinical cure on TBI. Methods TBI model of mice was made by freezing skull, the neural stem cells of transgenic mice of GFP were isolated, cultured and purifed, and then were transplanted into the mice model, different evaluating methods such as rotating fatigue testing machine were used to evaluate the nerve functions of mice, immunochemistry was used to detect the distribution of NSCs. Results Changes of praxiology and pathology of mice resulted from TBI, purified NSCs were proved to possess the generation capacity, and could differentiate to become neurons and astrocytes. The transplanted NSCs could not only integrated with the brain tissue, but also directly move to the injured brain tissue and take them place. Conclusion Ectogenic NCSs could survive in the injured brain tissue of mice and could promote the recovery of function of injured mice.