绿盲蝽对性信息素类似物和植物挥发物的触角电位反应

Electroantennogram responses of the green leaf bug,Lygus lucorum Meyer-Dr(Hemiptera:Miridae),to sex pheromone analogs and plant volatiles

为了更好地利用性信息素类似物与植物挥发物对绿盲蝽Lygus lucorum进行监测和生物防治,本研究采用电生理学方法测试了绿盲蝽成虫对9种性信息素类似物和12种植物挥发性物质的触角电位(electroantennogram,EAG)反应。结果表明:在测试的这21种化合物中,绿盲蝽对丁酸酯类化合物和绿叶气味物质的EAG反应较强;雄虫对性信息素类似物比雌虫更敏感,而雌虫对植物挥发物更敏感。其中性信息素类似物中,绿盲蝽对反-2-丁酸己烯酯的EAG反应最强;植物挥发物中,绿盲蝽对反-2-己烯醛的EAG反应最强,雌虫对反-2-己烯醇、反-2-己烯醛、顺-3-己烯醇等绿叶气味物质的EAG反应相对高于萜类化合物的反应。剂量反应曲线表明,在测定的浓度范围内,雄虫对反-2-丁酸己烯酯的反应比雌虫显著高,而对β-蒎烯的反应则比雌虫显著低。绿盲蝽成虫对性信息素类似物和植物挥发物的EAG反应存在的两性差异具有生态适应意义:雄虫对性信息素类似物更敏感将更有利于其寻找交配对象,而雌虫对绿叶气味物质更敏感将更有利于其寻找产卵寄主。

To better employ the alternative method for monitoring and controlling the green leaf bug,Lygus lucorum,with sex pheromone analogs and plant volatiles,electroantennogram （EAG） responses of adult L. lucorum to nine analogs of sex pheromone and twelve volatiles of plant were tested. The results indicated that EAG responses of L. lucorum to butyrates and green leaf volatiles （GLVs） were stronger than that to other compounds examined. Males were more sensitive to analogs of sex pheromone than females,while females more sensitive to plant volatiles. Among nine analogs of sex pheromone tested,（E）-2-hexenyl butyrate elicited the strongest EAG response. Among the plant volatiles tested,（E）-2-hexenal was the most stimulating. The females were more sensitive to such GLVs as （E）-2-hexenol,（E）-2-hexenal and （Z）-3-hexenol than to terpenoids. Dose response curves of EAG of L. lucorum showed that compared with females,males showed significantly stronger EAG responses to （E）-2-hexenyl butyrate at all tested concentrations,while significantly weaker EAG responses to β-pinene. Sensitivity differences between both sexes to sex pheromone analogs and plant volatiles suggest ecological adaptation of this species：the males are more sensitive to sex pheromone analogs,which will be helpful for their orientation towards females whereas the females are more sensitive to plant volatiles,which will be useful for their finding of hosts.