3-异丁基-1-甲基黄嘌呤对星形胶质细胞环磷酸鸟苷和胶质纤维酸性蛋白的影响

Influence of Phosphodiesterase Inhibitor 3-isobutyl-1-methylxathine on the Cyclic Guanosine Monophosphate and Glial Fibrillary Acidic Protein in Astrocytes

目的:探讨不同浓度的非特异性磷酸二酯酶(PDEs)抑制剂3-异丁基-1-甲基黄嘌呤(IBMX)对星形胶质细胞中环磷酸鸟苷(cGMP)浓度及胶质纤维酸性蛋白(GFAP)表达的影响。方法:体外纯化培养的SD乳鼠大脑皮质星形胶质细胞经鉴定(GFAP阳性)后随机分成8组,经不同浓度的IBMX干预后,用放射免疫法测定胞内cGMP浓度,免疫荧光测定细胞的GFAP荧光强度值。结果:IBMX 0.3和0.4 mmol·L^(-1)干预后,cGMP浓度及GFAP表达量与对照组相比差异均有统计学意义(P<0.05),两者水平随IBMX干预浓度的增加而增高,呈显著正相关(相关系数为0.95)。结论:IBMX可抑制PDEs的表达和cGMP降解。

Aim： To explore the effects of different concentrations of 3-isobutyl- 1-methylxathine（IBMX） on the concentration of cyclic guanosine monophosphate（cGMP） and expression of glial fibrillary acidic protein （GFAP） in astrocytes. Methods： The incubated and purified （identified by GFAP positive percent） astrocytes taken from cerebral cortex of neonatal SD rat were randomly divided into 8 groups. After the intervention of different concentrations of IBMX, the concentration of cGMP was determined by radioimmunoassay, and the expression of GFAP was measured by immunofluorescence-histochemistry. Results： IBMX could significantly increase the concentration of cGMP and the expression of GFAP, and there was a positive correlation （correlation coefficient is 0.95） between cGMP and GFAP. Conclusion： IBMX might play an important role in decreasing the expression of phosphodiesterase and inhibiting the degradation of cGMP.