应用生物素—亲和素系统提高检测脑脊液中囊虫抗体的敏感性

Using Biotin-Avidin System to Amplify Sensitivity of Detecting Cysticercus Antibodies in CSF

对21例脑囊虫病和65例非脑囊虫病患者脑脊液用ABC-ELISA法和常规ELISA法进行了检测。结果表明,21例脑囊虫病患者囊虫抗体阳性检出率分别为90.5％和81.0％,抗体几何平均滴度两法分别为1:66.66和1:28.87,差异有显著性(P<0.05),ABC—ELISA法的敏感性相当于常规ELISA法的2.3倍,有2例ABC-ELISA法为阳性,而常规ELISA法为假阴性。以上情况说明,ABC—ELISA法敏感性更高,并具有更少的假阴性,在抗体水平低的患者尤为敏感。65例非脑囊虫病患者两法均为阴性。

Avidin-Biotin-HRP Complex-ELISA (ABC-ELISA) and conventional ELISA for assaying of cerebraspinal fluid(CSF) cysticercus antibodies (Abs)were carried out in 21 cerebral cysticercosis patients and 65 noncysticercosis patients. Results showedthat in the 21 patients group, the CSF cysticercus Abs positive rate via ABC ELISA was 90.5%, while that via the conventional ELISA was 81.0%; in both immun oassays, the geometrical mean titers (GMT) of cysticercus Abs were 1:66. 66 and 1:28.87 respectively (P<0.05), the sensitivity of ABC-ELISA was about 2.3 times as high as that of the conventional ELISA. Tt was revealed that ABCELISA bore a more lower false negative rate, and comparing with the conventional ELISA was more sensitive in pati■nts with a low CSF cystic ercus A■s level. No false positiv■ reacti■s were observed in CSFs of the 65 non-cysticercosis patients by using ■ither assaying method.